000130394 001__ 130394
000130394 005__ 20240130142252.0
000130394 0247_ $$2doi$$a10.1093/nar/gks686
000130394 0248_ $$2sideral$$a78997
000130394 037__ $$aART-2012-78997
000130394 041__ $$aeng
000130394 100__ $$aGuallar, D.
000130394 245__ $$aExpression of endogenous retroviruses is negatively regulated by the pluripotency marker Rex1/Zfp42
000130394 260__ $$c2012
000130394 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130394 5203_ $$aRex1/Zfp42 is a Yy1 -related zinc-finger protein whose expression is frequently used to identify pluripotent stem cells. We show that depletion of Rex1 levels notably affected self-renewal of mouse embryonic stem (ES) cells in clonal assays, in the absence of evident differences in expression of marker genes for pluripotency or differentiation. By contrast, marked differences in expression of several endogenous retroviral elements (ERVs) were evident upon Rex1 depletion. We demonstrate association of REX1 to specific elements in chromatin-immunoprecipitation assays, most strongly to muERV-L and to a lower extent to IAP and musD elements. Rex1 regulates muERV-L expression in vivo , as we show altered levels upon transient gain-and-loss of Rex1 function in pre-implantation embryos. We also find REX1 can associate with the lysine-demethylase LSD1/KDM1A, suggesting they act in concert. Similar to REX1 binding to retrotransposable elements (REs) in ES cells, we also detected binding of the REX1 related proteins YY1 and YY2 to REs, although the binding preferences of the two proteins were slightly different. Altogether, we show that Rex1 regulates ERV expression in mouse ES cells and during pre-implantation development and suggest that Rex1 and its relatives have evolved as regulators of endogenous retroviral transcription.
000130394 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/B77$$9info:eu-repo/grantAgreement/ES/DGA/PI110-09$$9info:eu-repo/grantAgreement/ES/FIS/PI07119$$9info:eu-repo/grantAgreement/ES/IACS/PIPAMER-10-03$$9info:eu-repo/grantAgreement/ES/MEC/BFU2004-00467
000130394 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000130394 590__ $$a8.278$$b2012
000130394 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b27 / 288 = 0.094$$c2012$$dQ1$$eT1
000130394 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130394 700__ $$0(orcid)0000-0001-8655-9267$$aPérez-Palacios, R.
000130394 700__ $$0(orcid)0000-0002-0606-0763$$aCliment, M.$$uUniversidad de Zaragoza
000130394 700__ $$aMartínez-Abadía, I.
000130394 700__ $$aLarraga, A.
000130394 700__ $$aFernández-Juan, M.
000130394 700__ $$aVallejo, C.
000130394 700__ $$0(orcid)0000-0003-2014-2869$$aMuniesa, P.$$uUniversidad de Zaragoza
000130394 700__ $$aSchoorlemmer, J.
000130394 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000130394 773__ $$g40, 18 (2012), 8993-9007$$pNucleic acids res.$$tNucleic Acids Research$$x0305-1048
000130394 8564_ $$s1333630$$uhttps://zaguan.unizar.es/record/130394/files/texto_completo.pdf$$yVersión publicada
000130394 8564_ $$s3348836$$uhttps://zaguan.unizar.es/record/130394/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130394 909CO $$ooai:zaguan.unizar.es:130394$$particulos$$pdriver
000130394 951__ $$a2024-01-30-14:17:47
000130394 980__ $$aARTICLE