000130447 001__ 130447
000130447 005__ 20240125162930.0
000130447 0247_ $$2doi$$a10.1055/s-0028-1128146
000130447 0248_ $$2sideral$$a116143
000130447 037__ $$aART-2009-116143
000130447 041__ $$aeng
000130447 100__ $$aGómez, O.
000130447 245__ $$aExpression and Regulation of Insulin and the Glucose Transporter GLUT8 in the Testes of Diabetic Rats
000130447 260__ $$c2009
000130447 5203_ $$aDiabetes induces several malfunctions in male germ cells. The aim of this study was to analyze the levels and localization of the glucose transporter GLUT8 and insulin in the testes of rats induced to a diabetic status by a single dose of streptozotocin. One month after inducing diabetes, the GLUT8 immunoreactivity in diabetic rats was mainly located associated to the acrosomic system of spermatids, and at low levels in Leydig cells. Neither the immunohistochemical localization of this transporter nor its levels showed any difference when compared to control rats. Furthermore, it was observed that control rat testes expressed insulin, which was diffusely located in the cytoplasm of both Leydig cells and early elongated spermatids and concentrated in a cytoplasmic compartment in the more mature spermatids. Testicular insulin levels measured by western blot were reduced by more than half in diabetic rats, although the distribution of the hormone was unchanged. These results indicate that i) insulin is produced by testicular cells, ii) insulin is depleted by streptozotocin-induced diabetes, and iii) that insulin depletion and hyperglycemia do not regulate the expression of GLUT8 in testes. These results also suggest that testicular production of insulin could play a role in regulating spermatogenesis and/or glucose metabolism in these organs.
000130447 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130447 590__ $$a2.686$$b2009
000130447 591__ $$aENDOCRINOLOGY & METABOLISM$$b51 / 104 = 0.49$$c2009$$dQ2$$eT2
000130447 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130447 700__ $$aBallester, B.
000130447 700__ $$aRomero, A.
000130447 700__ $$aArnal, E.
000130447 700__ $$aAlmansa, I.
000130447 700__ $$aMiranda, M.
000130447 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, J.$$uUniversidad de Zaragoza
000130447 700__ $$aTerrado, J.
000130447 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDpto. Farmacología y Fisiolog.$$cÁrea Fisiología
000130447 773__ $$g41, 5 (2009), 343-349$$pHorm. Metab. Res.$$tHORMONE AND METABOLIC RESEARCH$$x0018-5043
000130447 8564_ $$s468877$$uhttps://zaguan.unizar.es/record/130447/files/texto_completo.pdf$$yVersión publicada
000130447 8564_ $$s2372676$$uhttps://zaguan.unizar.es/record/130447/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130447 909CO $$ooai:zaguan.unizar.es:130447$$particulos$$pdriver
000130447 951__ $$a2024-01-25-15:11:19
000130447 980__ $$aARTICLE