130448 20240125162930.0 doi 10.1177/1753425909104781 sideral 116144 ART-2009-116144 eng Mendoza, Carmen Lipopolysaccharide induces alteration of serotonin transporter in human intestinal epithelial cells 2009 Intestinal serotoninergic activity and serotonin transporter (SERT) function have been shown to be altered in intestinal inflammatory diseases. Serotonin (5-HT) plays a critical role in the regulation of gastrointestinal physiology. Activity of 5-HT depends on its extracellular availability, partly modulated by SERT that transports 5-HT into the cell. Lipopolysaccharide (LPS) is a component of Gram-negative bacteria outer membrane, which acts as a potent activator of the inflammatory system in the intestine. The aim of this work was to determine, in the enterocyte-like cell line Caco-2, whether LPS treatment affects serotoninergic activity by acting on SERT. The results demonstrate that LPS treatment diminishes SERT activity in a dose- and period-dependent way. The kinetic study shows that V<inf>max</inf> was significantly reduced after treatment with LPS. The LPS effect on 5-HT uptake was, in part, mediated by protein kinase C (PKC) activation. The molecular expression of SERT revealed that LPS treatment did not affect the mRNA level or the SERT protein content in cell homogenate. The level of SERT protein, however, was reduced on brush border membrane. The LPS effect might be due to an alteration of the intracellular traffic of SERT which may, in part, be mediated by PKC activity. ¬© SAGE Publications 2009. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.206 2009 MEDICINE, RESEARCH & EXPERIMENTAL 41 / 91 = 0.451 2009 Q2 T2 MICROBIOLOGY 54 / 95 = 0.568 2009 Q3 T2 BIOCHEMISTRY & MOLECULAR BIOLOGY 177 / 281 = 0.63 2009 Q3 T2 IMMUNOLOGY 87 / 126 = 0.69 2009 Q3 T3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Matheus, Nyurky (orcid)0000-0002-1605-0542 Iceta, Ruth Universidad de Zaragoza Mesonero, Jose E. Universidad de Zaragoza (orcid)0000-0003-4758-3998 Alcalde, Ana I. Universidad de Zaragoza (orcid)0000-0002-9935-927X 1005 410 Universidad de Zaragoza Dpto. Farmacología y Fisiolog. Área Fisiología 15, 4 (2009), 243-250 Innate immun. Innate Immunity 1753-4259 198077 http://zaguan.unizar.es/record/130448/files/texto_completo.pdf Versión publicada 902508 http://zaguan.unizar.es/record/130448/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130448 articulos driver 2024-01-25-15:11:30 ARTICLE