000130490 001__ 130490
000130490 005__ 20240130142254.0
000130490 0247_ $$2doi$$a10.3390/cells10051123
000130490 0248_ $$2sideral$$a126607
000130490 037__ $$aART-2021-126607
000130490 041__ $$aeng
000130490 100__ $$0(orcid)0000-0001-8655-9267$$aPérez-Palacios, R.$$uUniversidad de Zaragoza
000130490 245__ $$aYy2 in mouse preimplantation embryos and in embryonic stem cells
000130490 260__ $$c2021
000130490 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130490 5203_ $$aYin Yang 2 encodes a mammalian-specific transcription factor (YY2) that shares high homology in the zinc finger region with both YY1 and REX1/ZFP42, encoded by the Yin Yang 1 and Reduced Expression Protein 1/Zinc Finger Protein 42 gene, respectively. In contrast to the well-established roles of the latter two in gene regulation, X chromosome inactivation and binding to specific transposable elements (TEs), much less is known about YY2, and its presence during mouse preimplantation development has not been described. As it has been reported that mouse embryonic stem cells (mESC) cannot be propagated in the absence of Yy2, the mechanistic understanding of how Yy2 contributes to mESC maintenance remains only very partially characterized. We describe Yy2 expression studies using RT-PCR and staining with a high-affinity polyclonal serum in mouse embryos and mESC. Although YY2 is expressed during preimplantation development, its presence appears dispensable for developmental progress in vitro until formation of the blastocyst. Attenuation of Yy2 levels failed to alter either Zscan4 levels in two-cell embryos or IAP and MERVL levels at later preimplantation stages. In contrast to previous claims that constitutively expressed shRNA against Yy2 in mESC prohibited the propagation of mESC in culture, we obtained colonies generated from mESC with attenuated Yy2 levels. Concomitant with a decreased number of undifferentiated colonies, Yy2-depleted mESC expressed higher levels of Zscan4 but no differences in the expression of TEs or other pluripotency markers including Sox2, Oct4, Nanog and Esrrb were observed. These results confirm the contribution of Yy2 to the maintenance of mouse embryonic stem cells and show the preimplantation expression of YY2. These functions are discussed in relation to mammalian-specific functions of YY1 and REX1. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
000130490 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B138-10$$9info:eu-repo/grantAgreement/ES/DGA/C071-2014$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/B05-17D$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/B77$$9info:eu-repo/grantAgreement/ES/DGA/PI110-09$$9info:eu-repo/grantAgreement/ES/FIS/PI07119
000130490 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000130490 590__ $$a7.666$$b2021
000130490 591__ $$aCELL BIOLOGY$$b51 / 195 = 0.262$$c2021$$dQ2$$eT1
000130490 592__ $$a1.452$$b2021
000130490 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2021$$dQ1
000130490 594__ $$a6.7$$b2021
000130490 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130490 700__ $$0(orcid)0000-0002-0606-0763$$aCliment, M.$$uUniversidad de Zaragoza
000130490 700__ $$aSantiago-Arcos, J.
000130490 700__ $$aMacías-Redondo, S.
000130490 700__ $$aKlar, M.
000130490 700__ $$0(orcid)0000-0003-2014-2869$$aMuniesa, P.$$uUniversidad de Zaragoza
000130490 700__ $$aSchoorlemmer, J.
000130490 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000130490 773__ $$g10, 5 (2021), 1123 [16 pp.]$$pCells$$tCells$$x2073-4409
000130490 8564_ $$s1786370$$uhttps://zaguan.unizar.es/record/130490/files/texto_completo.pdf$$yVersión publicada
000130490 8564_ $$s2595380$$uhttps://zaguan.unizar.es/record/130490/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130490 909CO $$ooai:zaguan.unizar.es:130490$$particulos$$pdriver
000130490 951__ $$a2024-01-30-14:18:54
000130490 980__ $$aARTICLE