130502 20240125162931.0 doi 10.1089/ten.tec.2015.0062 sideral 92848 ART-2015-92848 eng Llobet, L. Universidad de Zaragoza (orcid)0000-0002-4379-1100 Side Effects of Culture Media Antibiotics on Cell Differentiation 2015 Besides the advance in scientific knowledge and the production of different compounds, cell culture can now be used to obtain cells for regenerative medicine. To avoid microbial contamination, antibiotics were usually incorporated into culture media. However, these compounds affect cell biochemistry and may modify the differentiation potential of cultured cells. To check this possibility, we grew human adipose tissue-derived stem cells and differentiated them to adipocyte with or without antibiotics commonly used in these culture protocols, such as a penicillin-streptomycin-amphotericin mix or gentamicin. We show that these antibiotics affect cell differentiation. Therefore, antibiotics should not be used in cell culture because aseptic techniques make these compounds unnecessary. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.174 2015 Bioengineering 2015 Q1 Medicine (miscellaneous) 2015 Q1 Biomedical Engineering 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Montoya, J. Universidad de Zaragoza (orcid)0000-0003-1770-6299 López-Gallardo, E. Universidad de Zaragoza (orcid)0000-0002-3217-1424 Ruiz-Pesini, E. Universidad de Zaragoza (orcid)0000-0002-0269-7337 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 21, 11 (2015), 1143-1147 Tissue eng. Part C Methods Tissue engineering. Part C Methods 1937-3384 211936 http://zaguan.unizar.es/record/130502/files/texto_completo.pdf Versión publicada 2621731 http://zaguan.unizar.es/record/130502/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130502 articulos driver 2024-01-25-15:15:52 ARTICLE