000130511 001__ 130511 000130511 005__ 20240125162931.0 000130511 0247_ $$2doi$$a10.1111/j.1365-2133.2005.06947.x 000130511 0248_ $$2sideral$$a129744 000130511 037__ $$aART-2006-129744 000130511 041__ $$aeng 000130511 100__ $$aGallardo, F. 000130511 245__ $$aPrimary cutaneous B-cell lymphoma (marginal zone) with prominent T-cell component and aberrant dual (T and B) genotype; diagnostic usefulness of laser-capture microdissection 000130511 260__ $$c2006 000130511 5203_ $$aThe presence of a dominant B- or T-cell clone is an important diagnostic criterion for distinguishing cutaneous lymphomas from lymphoid reactive infiltrates. Rarely, a combined B- and T-cell rearrangement can be detected from a single sample. In such instances, genotypic analysis does not permit differentiation of the coexistence of a T- and B-cell lymphoma from a single clone harbouring a monoclonal rearrangement for both immunoglobulin heavy chain and T-cell receptor genes. We herein report a case of a skin tumour consistent with a dense cutaneous lymphoid infiltrate showing a double prominent B- and T-cell component. A dual B- and T-cell clonality was detected by polymerase chain reaction from whole-tissue DNA sample. Genotypic analysis with DNA, obtained after laser-assisted microdissection from the B-cell population, again showed both T- and B-cell monoclonal rearrangements. Conversely, the microdissected T-cell population did not reveal a clonal pattern. The diagnosis of cutaneous B-cell lymphoma with a dual B- and T-cell genotype was established. This description illustrates the diagnostic usefulness of laser-capture microdissection in cutaneous lymphomas presenting dual genotype. 000130511 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000130511 590__ $$a3.334$$b2006 000130511 591__ $$aDERMATOLOGY$$b2 / 39 = 0.051$$c2006$$dQ1$$eT1 000130511 655_4 $$ainfo:eu-repo/semantics/article 000130511 700__ $$aPujol, R.M. 000130511 700__ $$aBellosillo, B. 000130511 700__ $$aFerrer, D. 000130511 700__ $$0(orcid)0000-0003-2078-8205$$aGarcía, M. 000130511 700__ $$aBarranco, C. 000130511 700__ $$aPlanagum\u00e0, M. 000130511 700__ $$aSerrano, S. 000130511 773__ $$g154, 1 (2006), 162-166$$pBr. j. dermatol.$$tBritish Journal of Dermatology$$x0007-0963 000130511 8564_ $$s710973$$uhttps://zaguan.unizar.es/record/130511/files/texto_completo.pdf 000130511 8564_ $$s2358280$$uhttps://zaguan.unizar.es/record/130511/files/texto_completo.jpg?subformat=icon$$xicon 000130511 909CO $$ooai:zaguan.unizar.es:130511$$particulos$$pdriver 000130511 951__ $$a2024-01-25-15:16:37 000130511 980__ $$aARTICLE