130511 20240125162931.0 doi 10.1111/j.1365-2133.2005.06947.x sideral 129744 ART-2006-129744 eng Gallardo, F. Primary cutaneous B-cell lymphoma (marginal zone) with prominent T-cell component and aberrant dual (T and B) genotype; diagnostic usefulness of laser-capture microdissection 2006 The presence of a dominant B- or T-cell clone is an important diagnostic criterion for distinguishing cutaneous lymphomas from lymphoid reactive infiltrates. Rarely, a combined B- and T-cell rearrangement can be detected from a single sample. In such instances, genotypic analysis does not permit differentiation of the coexistence of a T- and B-cell lymphoma from a single clone harbouring a monoclonal rearrangement for both immunoglobulin heavy chain and T-cell receptor genes. We herein report a case of a skin tumour consistent with a dense cutaneous lymphoid infiltrate showing a double prominent B- and T-cell component. A dual B- and T-cell clonality was detected by polymerase chain reaction from whole-tissue DNA sample. Genotypic analysis with DNA, obtained after laser-assisted microdissection from the B-cell population, again showed both T- and B-cell monoclonal rearrangements. Conversely, the microdissected T-cell population did not reveal a clonal pattern. The diagnosis of cutaneous B-cell lymphoma with a dual B- and T-cell genotype was established. This description illustrates the diagnostic usefulness of laser-capture microdissection in cutaneous lymphomas presenting dual genotype. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 3.334 2006 DERMATOLOGY 2 / 39 = 0.051 2006 Q1 T1 info:eu-repo/semantics/article Pujol, R.M. Bellosillo, B. Ferrer, D. García, M. (orcid)0000-0003-2078-8205 Barranco, C. Planagum\u00e0, M. Serrano, S. 154, 1 (2006), 162-166 Br. j. dermatol. British Journal of Dermatology 0007-0963 710973 http://zaguan.unizar.es/record/130511/files/texto_completo.pdf 2358280 http://zaguan.unizar.es/record/130511/files/texto_completo.jpg?subformat=icon icon oai:zaguan.unizar.es:130511 articulos driver 2024-01-25-15:16:37 ARTICLE