000130530 001__ 130530
000130530 005__ 20240125162931.0
000130530 0247_ $$2doi$$a10.1002/jbm.a.32988
000130530 0248_ $$2sideral$$a67660
000130530 037__ $$aART-2011-67660
000130530 041__ $$aeng
000130530 100__ $$0(orcid)0000-0003-2410-5678$$aOchoa Garrido, Ignacio$$uUniversidad de Zaragoza
000130530 245__ $$aMechanical properties of cross-linked collagen meshes after human adipose derived stromal cells seeding
000130530 260__ $$c2011
000130530 5203_ $$aThe main goal of this study was to evaluate the potential of collagen meshes derived from porcine dermis as scaffolds for repairing pelvic organ prolapses. Mechanical properties of collagen meshes with different cross-linking percentages before and after Adipose Derived Stromal Cells (ADSC) seeding were studied as well as the cell-scaffold interaction. Uniaxial tensile tests of the collagen meshes with three different cross-linking percentages (full-, partial-, and noncross-linked) were carried out along orthogonal directions. Their mechanical properties were studied with the same tests before and after seeding with human derived adipose stem cells (ADSC) after 1 and 7 days. Histological analyses were performed to determine adhesion and proliferation of ADSC. Significant differences in mechanical properties of the unseeded meshes were observed between each orthogonal direction independently of the cross-linking percentage. A better cell adhesion rate was observed in the cross-linked meshes. An increase in the mechanical properties after cell seeding was observed with a direct relation with the degree of cross-linking. All meshes analyzed showed a marked anisotropy that should be taken into account during the surgical procedure. The cross-linking treatment increased cell adhesion and the mechanical properties of the collagen meshes after seeding. These results suggest that the mechanical properties of this type of collagen mesh could be useful as scaffolds for repair of pelvic organ prolapse.
000130530 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130530 590__ $$a2.625$$b2011
000130530 591__ $$aENGINEERING, BIOMEDICAL$$b17 / 72 = 0.236$$c2011$$dQ1$$eT1
000130530 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b11 / 25 = 0.44$$c2011$$dQ2$$eT2
000130530 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130530 700__ $$0(orcid)0000-0002-0664-5024$$aPeña Baquedano, Estefania$$uUniversidad de Zaragoza
000130530 700__ $$aAndreu, Enrique J.
000130530 700__ $$aPerez-Ilzarbe, Maitane
000130530 700__ $$aRobles, Jose E
000130530 700__ $$0(orcid)0000-0002-7854-8856$$aAlcaine Gonzalez, Clara
000130530 700__ $$aLopez, Tania
000130530 700__ $$aProsper, Felipe
000130530 700__ $$0(orcid)0000-0001-8741-6452$$aDoblare Castellano, Manuel$$uUniversidad de Zaragoza
000130530 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000130530 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000130530 773__ $$g96A, 2 (2011), 341-348$$pJ. Biomed. Mater. Res. Part A$$tJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A$$x1549-3296
000130530 8564_ $$s1840805$$uhttps://zaguan.unizar.es/record/130530/files/texto_completo.pdf$$yVersión publicada
000130530 8564_ $$s2611001$$uhttps://zaguan.unizar.es/record/130530/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130530 909CO $$ooai:zaguan.unizar.es:130530$$particulos$$pdriver
000130530 951__ $$a2024-01-25-15:17:43
000130530 980__ $$aARTICLE