000130531 001__ 130531
000130531 005__ 20240125162931.0
000130531 0247_ $$2doi$$a10.3109/03008207.2012.762360
000130531 0248_ $$2sideral$$a81870
000130531 037__ $$aART-2013-81870
000130531 041__ $$aeng
000130531 100__ $$aSanz-Ramos, P.
000130531 245__ $$aResponse of sheep chondrocytes to changes in substrate stiffness from 2 to 20 pa: Effect of cell passaging
000130531 260__ $$c2013
000130531 5203_ $$aAim: The influence of culture substrate stiffness (in the kPa range) on chondrocyte behavior has been described. Here we describe the response to variations in substrate stiffness in a soft range (2–20 Pa), as it may play a role in understanding cartilage physiopathology.
Methods: We developed a system for cell culture in substrates with different elastic moduli using collagen hydrogels and evaluated chondrocytes after 2, 4, and 7 days in monolayer and three-dimensional (3D) cultures. Experiments were performed in normoxia and hypoxia in order to describe the effect of a low oxygen environment on chondrocytes. Finally, we also evaluated if dedifferentiated cells preserve the capacity for mechanosensing.
Results: Chondrocytes showed less proliferating activity when cultured in monolayer in the more compliant substrates. Expression of the cartilage markers Aggrecan (Acan), type II collagen (Col2a1), and Sox9 was upregulated in the less stiff gels (both in monolayer and in 3D culture). Stiffer gels induced an organization of the actin cytoskeleton that correlated with the loss of a chondrocyte phenotype. When cells were cultured in hypoxia, we observed changes in the cellular response that were mediated by HIF-1α. Results in 3D hypoxia cultures were opposite to those found in normoxia, but remained unchanged in monolayer hypoxic experiments. Similar results were found for dedifferentiated cells.
Conclusions: Chondrocytes respond differently according to the stiffness of the substrate. This response depends greatly on the oxygen environment and on whether the chondrocyte is embedded or grown onto the hydrogel, since mechanosensing capacity was not lost with cell expansion.
000130531 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130531 590__ $$a1.982$$b2013
000130531 591__ $$aORTHOPEDICS$$b24 / 66 = 0.364$$c2013$$dQ2$$eT2
000130531 591__ $$aCELL BIOLOGY$$b142 / 184 = 0.772$$c2013$$dQ4$$eT3
000130531 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130531 700__ $$aMora, G.
000130531 700__ $$aVicente-Pascual, M.
000130531 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, I.$$uUniversidad de Zaragoza
000130531 700__ $$0(orcid)0000-0002-7854-8856$$aAlcaine, C.
000130531 700__ $$0(orcid)0000-0002-6600-1618$$aMoreno, R.$$uUniversidad de Zaragoza
000130531 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, M.$$uUniversidad de Zaragoza
000130531 700__ $$aIzal-Azcárate, I.
000130531 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000130531 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000130531 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000130531 773__ $$g54, 3 (2013), 159-166$$pConnect. tissue res.$$tCONNECTIVE TISSUE RESEARCH$$x0300-8207
000130531 8564_ $$s891619$$uhttps://zaguan.unizar.es/record/130531/files/texto_completo.pdf$$yVersión publicada
000130531 8564_ $$s894964$$uhttps://zaguan.unizar.es/record/130531/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130531 909CO $$ooai:zaguan.unizar.es:130531$$particulos$$pdriver
000130531 951__ $$a2024-01-25-15:17:49
000130531 980__ $$aARTICLE