000130544 001__ 130544
000130544 005__ 20240126182118.0
000130544 0247_ $$2doi$$a10.5301/ijao.5000096
000130544 0248_ $$2sideral$$a136571
000130544 037__ $$aART-2012-136571
000130544 041__ $$aeng
000130544 100__ $$aChouhan, M.
000130544 245__ $$aHepatocyte labeling with Tc-99m-gsa: a potential non-invasive technique for tracking cell transplantation
000130544 260__ $$c2012
000130544 5203_ $$aBackground: Hepatocyte transplantation is a promising alternative to orthotopic liver transplantation, however, the fate of transplanted hepatocytes is not well defined. 99mTc-galactosyl-serum albumin (99mTc-GSA) is a clinical scintigraphic agent which is specifically taken up by the hepatocyte asialoglycoprotein receptor (ASGPR). Aims: To investigate labeling of fresh and cryopreserved human hepatocytes and fresh rat hepatocytes in vitro using 99mTc-GSA Methods: Human and rat hepatocytes were isolated from liver tissue by collagenase perfusion. The ASGPR were characterized using immunohistochemistry and RT-PCR. Hepatocytes were incubated with 99mTc-GSA in suspension at 4°C and 37°C. Cell viability and function was determined using cell mitochondrial dehydrogenase (MTS) and sulphorhodamine B (SRB) assays. Results: Fresh and cryopreserved human hepatocytes expressed the ASGPR. Incubation of hepatocytes in suspension with 99mTc-GSA reduced the viability of hepatocytes, but this was similar to unlabeled control cells. Greater loss of viability was seen on incubation at 37°C compared to 4°C, but there was a significantly greater uptake of 99mTc-GSA at the physiological temperature (6.6 ± SE 0.6-fold increase, p<0.05) consistent with ASGPR-mediated endocytosis. MTS and SRB assays were not significantly affected by labeling with 99mTc-GSA in all three cell types. A mean of 18.5% of the radioactivity was released over 120 min when 99mTc-GSA - labeled hepatocytes were shaken in vitro at 37°C. Conclusions: Human and rat hepatocytes can be labeled with 99mTc-GSA, which may have potential application for in vivo imaging after hepatocyte transplantation.
000130544 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130544 590__ $$a1.759$$b2012
000130544 591__ $$aENGINEERING, BIOMEDICAL$$b35 / 76 = 0.461$$c2012$$dQ2$$eT2
000130544 591__ $$aTRANSPLANTATION$$b16 / 26 = 0.615$$c2012$$dQ3$$eT2
000130544 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130544 700__ $$aPuppi, J.
000130544 700__ $$0(orcid)0000-0001-8056-5236$$aSolanas, E.
000130544 700__ $$aMitry, R.
000130544 700__ $$aDhawan, A.
000130544 700__ $$aHughes, R.D.
000130544 773__ $$g35, 6 (2012), 450-457$$pInt. j. artif. organs$$tINTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS$$x0391-3988
000130544 8564_ $$s309926$$uhttps://zaguan.unizar.es/record/130544/files/texto_completo.pdf$$yVersión publicada
000130544 8564_ $$s2247184$$uhttps://zaguan.unizar.es/record/130544/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130544 909CO $$ooai:zaguan.unizar.es:130544$$particulos$$pdriver
000130544 951__ $$a2024-01-26-18:17:51
000130544 980__ $$aARTICLE