130544 20240126182118.0 doi 10.5301/ijao.5000096 sideral 136571 ART-2012-136571 eng Chouhan, M. Hepatocyte labeling with Tc-99m-gsa: a potential non-invasive technique for tracking cell transplantation 2012 Background: Hepatocyte transplantation is a promising alternative to orthotopic liver transplantation, however, the fate of transplanted hepatocytes is not well defined. 99mTc-galactosyl-serum albumin (99mTc-GSA) is a clinical scintigraphic agent which is specifically taken up by the hepatocyte asialoglycoprotein receptor (ASGPR). Aims: To investigate labeling of fresh and cryopreserved human hepatocytes and fresh rat hepatocytes in vitro using 99mTc-GSA Methods: Human and rat hepatocytes were isolated from liver tissue by collagenase perfusion. The ASGPR were characterized using immunohistochemistry and RT-PCR. Hepatocytes were incubated with 99mTc-GSA in suspension at 4°C and 37°C. Cell viability and function was determined using cell mitochondrial dehydrogenase (MTS) and sulphorhodamine B (SRB) assays. Results: Fresh and cryopreserved human hepatocytes expressed the ASGPR. Incubation of hepatocytes in suspension with 99mTc-GSA reduced the viability of hepatocytes, but this was similar to unlabeled control cells. Greater loss of viability was seen on incubation at 37°C compared to 4°C, but there was a significantly greater uptake of 99mTc-GSA at the physiological temperature (6.6 ± SE 0.6-fold increase, p<0.05) consistent with ASGPR-mediated endocytosis. MTS and SRB assays were not significantly affected by labeling with 99mTc-GSA in all three cell types. A mean of 18.5% of the radioactivity was released over 120 min when 99mTc-GSA - labeled hepatocytes were shaken in vitro at 37°C. Conclusions: Human and rat hepatocytes can be labeled with 99mTc-GSA, which may have potential application for in vivo imaging after hepatocyte transplantation. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 1.759 2012 ENGINEERING, BIOMEDICAL 35 / 76 = 0.461 2012 Q2 T2 TRANSPLANTATION 16 / 26 = 0.615 2012 Q3 T2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Puppi, J. Solanas, E. (orcid)0000-0001-8056-5236 Mitry, R. Dhawan, A. Hughes, R.D. 35, 6 (2012), 450-457 Int. j. artif. organs INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS 0391-3988 309926 http://zaguan.unizar.es/record/130544/files/texto_completo.pdf Versión publicada 2247184 http://zaguan.unizar.es/record/130544/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130544 articulos driver 2024-01-26-18:17:51 ARTICLE