000130639 001__ 130639
000130639 005__ 20240208144955.0
000130639 0248_ $$2sideral$$a73082
000130639 0247_ $$2doi$$a10.1016/j.scr.2011.02.005
000130639 037__ $$aART-2011-73082
000130639 041__ $$aeng
000130639 100__ $$aGarcia-Tuñon, I.
000130639 245__ $$aAssociation of Rex-1 to target genes supports its interaction with Polycomb function
000130639 260__ $$c2011
000130639 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130639 5203_ $$aRex-1/Zfp42 displays a remarkably restricted pattern of expression in preimplantation embryos, primary spermatocytes, and undifferentiated mouse embryonic stem (ES) cells and is frequently used as a marker gene for pluripotent stem cells. To understand the role of Rex-1 in selfrenewal and pluripotency, we used Rex-1 association as a measure to identify potential target genes, and carried out chromatin-immunoprecipitation assays in combination with gene specific primers to identify genomic targets Rex-1 associates with. We find association of Rex-1 to several genes described previously as bivalently marked regulators of differentiation and development, whose repression in mouse embryonic stem (ES) cells is Polycomb Group-mediated, and controlled directly by Ring1A/B. To substantiate the hypothesis that Rex-1 contributes to gene regulation by PcG, we demonstrate interactions of Rex-1 and YY2 (a close relative of YY1) with Ring1 proteins and the PcG-associated proteins RYBP and YAF2, in line with interactions reported previously for YY1. We also demonstrate the presence of Rex-1 protein in both trophectoderm and Inner Cell Mass of the mouse blastocyst and in both ES and in trophectoderm stem (TS) cells. In TS cells, we were unable to demonstrate association of Rex-1 to the genes it associates with in ES cells, suggesting that association may be cell-type specific. Rex-1 might fine-tune pluripotency in ES cells by modulating Polycomb-mediated gene regulation.
000130639 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130639 590__ $$a5.127$$b2011
000130639 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b18 / 158 = 0.114$$c2011$$dQ1$$eT1
000130639 591__ $$aCELL & TISSUE ENGINEERING$$b3 / 12 = 0.25$$c2011$$dQ1$$eT1
000130639 591__ $$aCELL BIOLOGY$$b48 / 179 = 0.268$$c2011$$dQ2$$eT1
000130639 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130639 700__ $$aGuallar, D.
000130639 700__ $$aAlonso-Martin, S.
000130639 700__ $$aBenito, A.A.
000130639 700__ $$aBenítez-Lázaro, A.
000130639 700__ $$0(orcid)0000-0001-8655-9267$$aPérez-Palacios, R.
000130639 700__ $$0(orcid)0000-0003-2014-2869$$aMuniesa, P.$$uUniversidad de Zaragoza
000130639 700__ $$0(orcid)0000-0002-0606-0763$$aCliment, M.$$uUniversidad de Zaragoza
000130639 700__ $$aSánchez, M.
000130639 700__ $$aVidal, M.
000130639 700__ $$aSchoorlemmer, J.
000130639 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000130639 773__ $$g7, 1 (2011), 1-16$$pStem Cell Research$$tSTEM CELL RESEARCH$$x1873-5061
000130639 8564_ $$s1489306$$uhttps://zaguan.unizar.es/record/130639/files/texto_completo.pdf$$yVersión publicada
000130639 8564_ $$s1062403$$uhttps://zaguan.unizar.es/record/130639/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130639 909CO $$ooai:zaguan.unizar.es:130639$$particulos$$pdriver
000130639 951__ $$a2024-02-08-14:46:57
000130639 980__ $$aARTICLE