000130640 001__ 130640 000130640 005__ 20240131193421.0 000130640 0247_ $$2doi$$a10.1089/scd.2012.0211 000130640 0248_ $$2sideral$$a80483 000130640 037__ $$aART-2013-80483 000130640 041__ $$aeng 000130640 100__ $$0(orcid)0000-0002-0606-0763$$aCliment, M. 000130640 245__ $$aFunctional analysis of rex1 during preimplantation development 000130640 260__ $$c2013 000130640 5203_ $$aRex1/Zfp42 is a nuclear protein that is highly conserved in mammals, and widely used as an embryonic stem (ES) cell marker. Although Rex1 expression is associated with enhanced pluripotency, loss-of-function models recently described do not exhibit major phenotypes, and both preimplantation development and ES cell derivation appear normal in the absence of Rex1. To better understand the functional role of Rex1, we examined the expression and localization of Rex1 during preimplantation development. Our studies indicated that REX1 is expressed at all stages during mouse preimplantation development, with a mixed pattern of nuclear, perinuclear, and cytoplasmic localization. Chromatin association seemed to be altered in 8-cell embryos, and in the blastocyst, we found REX1 localized almost exclusively in the nucleus. A functional role for Rex1 in vivo was assessed by gain- and loss-of-function approaches. Embryos with attenuated levels of Rex1 after injection of zygotes with siRNAs did not exhibit defects in preimplantation development in vitro. In contrast, overexpression of Rex1 interfered with cleavage divisions and with proper blastocyst development, although we failed to detect alterations in the expression of lineage and pluripotency markers. Rex1 gain- and loss-of-function did alter the expression levels of Zscan4, an important regulator of preimplantation development and pluripotency. Our results suggest that Rex1 plays a role during preimplantation development. They are compatible with a role for Rex1 during acquisition of pluripotency in the blastocyst. 000130640 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000130640 590__ $$a4.202$$b2013 000130640 591__ $$aHEMATOLOGY$$b15 / 68 = 0.221$$c2013$$dQ1$$eT1 000130640 591__ $$aTRANSPLANTATION$$b3 / 26 = 0.115$$c2013$$dQ1$$eT1 000130640 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b21 / 122 = 0.172$$c2013$$dQ1$$eT1 000130640 591__ $$aCELL & TISSUE ENGINEERING$$b6 / 18 = 0.333$$c2013$$dQ2$$eT2 000130640 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000130640 700__ $$aAlonso-Martin, S. 000130640 700__ $$0(orcid)0000-0001-8655-9267$$aPerez-Palacios, R. 000130640 700__ $$aGuallar, D. 000130640 700__ $$aBenito, A.A. 000130640 700__ $$aLarraga, A. 000130640 700__ $$aFernandez-Juan, M. 000130640 700__ $$aSanz, M. 000130640 700__ $$aDe Diego, A. 000130640 700__ $$aSeisdedos, M.T. 000130640 700__ $$0(orcid)0000-0003-2014-2869$$aMuniesa, P.$$uUniversidad de Zaragoza 000130640 700__ $$aSchoorlemmer, J. 000130640 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com 000130640 773__ $$g22, 3 (2013), 459-472$$pStem Cells Dev.$$tSTEM CELLS AND DEVELOPMENT$$x1547-3287 000130640 8564_ $$s1004089$$uhttps://zaguan.unizar.es/record/130640/files/texto_completo.pdf$$yVersión publicada 000130640 8564_ $$s2854200$$uhttps://zaguan.unizar.es/record/130640/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000130640 909CO $$ooai:zaguan.unizar.es:130640$$particulos$$pdriver 000130640 951__ $$a2024-01-31-19:29:53 000130640 980__ $$aARTICLE