000130719 001__ 130719
000130719 005__ 20240131193423.0
000130719 0247_ $$2doi$$a10.7554/eLife.11418
000130719 0248_ $$2sideral$$a136554
000130719 037__ $$aART-2016-136554
000130719 041__ $$aeng
000130719 100__ $$aWalter, Marius
000130719 245__ $$aAn epigenetic switch ensures transposon repression upon dynamic loss of DNA methylation in embryonic stem cells
000130719 260__ $$c2016
000130719 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130719 5203_ $$aDNA methylation is extensively remodeled during mammalian gametogenesis and embryogenesis. Most transposons become hypomethylated, raising the question of their regulation in the absence of DNA methylation. To reproduce a rapid and extensive demethylation, we subjected mouse ES cells to chemically defined hypomethylating culture conditions. Surprisingly, we observed two phases of transposon regulation. After an initial burst of de-repression, various transposon families were efficiently re-silenced. This was accompanied by a reconfiguration of the repressive chromatin landscape: while H3K9me3 was stable, H3K9me2 globally disappeared and H3K27me3 accumulated at transposons. Interestingly, we observed that H3K9me3 and H3K27me3 occupy different transposon families or different territories within the same family, defining three functional categories of adaptive chromatin responses to DNA methylation loss. Our work highlights that H3K9me3 and, most importantly, polycomb-mediated H3K27me3 chromatin pathways can secure the control of a large spectrum of transposons in periods of intense DNA methylation change, ensuring longstanding genome stability.
000130719 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000130719 590__ $$a7.725$$b2016
000130719 591__ $$aBIOLOGY$$b4 / 84 = 0.048$$c2016$$dQ1$$eT1
000130719 592__ $$a7.296$$b2016
000130719 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000130719 593__ $$aNeuroscience (miscellaneous)$$c2016$$dQ1
000130719 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000130719 593__ $$aImmunology and Microbiology (miscellaneous)$$c2016$$dQ1
000130719 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130719 700__ $$aTeissandier, Aurélie
000130719 700__ $$0(orcid)0000-0001-8655-9267$$aPérez-Palacios, Raquel
000130719 700__ $$aBourc'his, Déborah
000130719 773__ $$g5 (2016), e11418 [30 pp.]$$peLife (Cambridge)$$teLife$$x2050-084X
000130719 8564_ $$s5159111$$uhttps://zaguan.unizar.es/record/130719/files/texto_completo.pdf$$yVersión publicada
000130719 8564_ $$s2489970$$uhttps://zaguan.unizar.es/record/130719/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130719 909CO $$ooai:zaguan.unizar.es:130719$$particulos$$pdriver
000130719 951__ $$a2024-01-31-19:30:41
000130719 980__ $$aARTICLE