130737 20240201145617.0 doi 10.1016/j.jpba.2016.02.033 sideral 93999 ART-2016-93999 eng Carrizo, D. (orcid)0000-0003-1568-4591 Direct screening of tobacco indicators in urine and saliva by Atmospheric Pressure Solid Analysis Probe coupled to quadrupole-time of flight mass spectrometry (ASAP-MS-Q-TOF-) 2016 Access copy available to the general public Unrestricted A new screening method has been explored for direct analysis of tobacco smoke biomarkers in biological matrices (i.e., saliva and urine). Single run analysis using Atmospheric pressure Solid Analysis Probe (ASAP) and high resolution mass spectrometry with quadrupole and time of flight detector has been applied directly to some biological samples (i.e., urine and saliva), providing a fast, efficient and sensitive method of identification. The method has been applied to saliva and urine samples from heavy tobacco smokers for exposure studies. Nicotine itself, nicotine metabolites (i.e., cotinine, trans-3''-hydroxycotinine, nicotine-N-glucuronide) and other related tobacco smoke toxic compounds (i.e., NNK 4-methyl(nitroso)amino]-1-(3-pyridinyl)-1-butanone, anatabine) were found in the analyzed samples. The identification of compounds was confirmed by ultrahigh performance liquid chromatography with MS-triple quadrupole detector after sample treatment. Different temporal trends and biomarkers behavior have been found in time series related samples. Both methods are compared for screening of these biological matrices. info:eu-repo/grantAgreement/ES/DGA/T10 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.255 2016 CHEMISTRY, ANALYTICAL 18 / 76 = 0.237 2016 Q1 T1 PHARMACOLOGY & PHARMACY 70 / 256 = 0.273 2016 Q2 T1 0.92 2016 Pharmaceutical Science 2016 Q1 Analytical Chemistry 2016 Q1 Spectroscopy 2016 Q2 Clinical Biochemistry 2016 Q2 Drug Discovery 2016 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Nerín, I. Universidad de Zaragoza (orcid)0000-0001-6752-1992 Domeño, C. Universidad de Zaragoza (orcid)0000-0001-8893-0963 Alfaro, P. Universidad de Zaragoza (orcid)0000-0002-3703-7902 Nerín, C. Universidad de Zaragoza (orcid)0000-0003-2685-5739 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 2009 X Universidad de Zaragoza Dpto. Química Analítica Proy. investigación HVA 124 (2016), 149-156 J. pharm. biomed. anal. Journal of Pharmaceutical and Biomedical Analysis 0731-7085 1343050 http://zaguan.unizar.es/record/130737/files/texto_completo.pdf Postprint 1678929 http://zaguan.unizar.es/record/130737/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:130737 articulos driver 2024-02-01-14:51:53 ARTICLE