000130758 001__ 130758
000130758 005__ 20240131210810.0
000130758 0247_ $$2doi$$a10.1128/AAC.00517-12
000130758 0248_ $$2sideral$$a80667
000130758 037__ $$aART-2013-80667
000130758 041__ $$aeng
000130758 100__ $$aSecka,O.
000130758 245__ $$aAntimicrobial susceptibility and resistance patterns among helicobacter pylori strains from the gambia, west africa
000130758 260__ $$c2013
000130758 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130758 5203_ $$aHelicobacter pylori is a globally important and genetically diverse gastric pathogen that infects most people in developing countries. Eradication efforts are complicated by antibiotic resistance, which varies in frequency geographically. There are very few data on resistance in African strains. Sixty-four Gambian H. pylori strains were tested for antibiotic susceptibility. The role of rdxA in metronidazole (Mtz) susceptibility was tested by DNA transformation and sequencing; RdxA protein variants were interpreted in terms of RdxA structure. Forty-four strains (69%) were resistant to at least 8 μg of Mtz/ml. All six strains from infants, but only 24% of strains from adults, were sensitive (P = 0.0031). Representative Mtz-resistant (Mtzr) strains were rendered Mtz susceptible (Mtzs) by transformation with a functional rdxA gene; conversely, Mtzs strains were rendered Mtzr by rdxA inactivation. Many mutations were found by Gambian H. pylori rdxA sequencing; mutations that probably inactivated rdxA in Mtzr strains were identified and explained using RdxA protein's structure. All of the strains were sensitive to clarithromycin and erythromycin. Amoxicillin and tetracycline resistance was rare. Sequence analysis indicated that most tetracycline resistance, when found, was not due to 16S rRNA gene mutations. These data suggest caution in the use of Mtz-based therapies in The Gambia. The increasing use of macrolides against respiratory infections in The Gambia calls for continued antibiotic susceptibility monitoring. The rich variety of rdxA mutations that we found will be useful in further structure-function studies of RdxA, the enzyme responsible for Mtz susceptibility in this important pathogen.
000130758 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000130758 590__ $$a4.451$$b2013
000130758 591__ $$aPHARMACOLOGY & PHARMACY$$b27 / 255 = 0.106$$c2013$$dQ1$$eT1
000130758 591__ $$aMICROBIOLOGY$$b21 / 118 = 0.178$$c2013$$dQ1$$eT1
000130758 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130758 700__ $$aBerg,D. E.
000130758 700__ $$aAntonio,M.
000130758 700__ $$aCorrah,T.
000130758 700__ $$aTapgun,M.
000130758 700__ $$aWalton,R.
000130758 700__ $$aThomas,V.
000130758 700__ $$0(orcid)0000-0002-1896-7805$$aGalano,J. J.$$uUniversidad de Zaragoza
000130758 700__ $$0(orcid)0000-0002-2879-9200$$aSancho,J.$$uUniversidad de Zaragoza
000130758 700__ $$aAdegbola,R. A.
000130758 700__ $$aThomas,J. E.
000130758 7102_ $$11002$$2X$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cProy. investigación DEA
000130758 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000130758 773__ $$g57, 3 (2013), 1231-1237$$pAntimicrob. agents chemother.$$tAntimicrobial Agents and Chemotherapy$$x0066-4804
000130758 8564_ $$s294378$$uhttps://zaguan.unizar.es/record/130758/files/texto_completo.pdf$$yVersión publicada
000130758 8564_ $$s3333367$$uhttps://zaguan.unizar.es/record/130758/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130758 909CO $$ooai:zaguan.unizar.es:130758$$particulos$$pdriver
000130758 951__ $$a2024-01-31-19:18:31
000130758 980__ $$aARTICLE