000130774 001__ 130774
000130774 005__ 20240202150202.0
000130774 0247_ $$2doi$$a10.1016/j.anireprosci.2020.106539
000130774 0248_ $$2sideral$$a118989
000130774 037__ $$aART-2020-118989
000130774 041__ $$aeng
000130774 100__ $$0(orcid)0000-0002-7857-9732$$aLuño, V.$$uUniversidad de Zaragoza
000130774 245__ $$aColloid centrifugation reduces bacterial load in chilled dog semen
000130774 260__ $$c2020
000130774 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130774 5203_ $$aConventional semen extenders contain antibiotics to prevent bacterial growth. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage. Twenty-four ejaculates were obtained from healthy dogs by digital manipulation. Samples were diluted in Tris-citrate-fructose extender without antibiotics and divided into two treatment groups: SLC-selected samples and unselected samples. Sperm motility (CASA), viability and acrosome integrity (PI/FITC-PNA) as well as bacterial load of each microorganism species (colony-forming units/mL) were assessed at 0 and 48 h of storage at 4 °C. Results indicate SLC-selected dog spermatozoa have greater percentages of motility, viability and acrosome integrity (P < 0.05). Bacterial growth in SLC sperm samples was less (P < 0.05) than unselected samples. Removal of individual bacterial species varied from 91 % to 98 % for Escherichia coli (91.62 %), Streptococcus spp. (98.18 %), Staphylococcus spp.(95.33 %) and Pseudomonas spp. (92.50 %). In conclusion, the use of SLC with Canicoll has the potential to decrease bacterial load in chilled dog semen.
000130774 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/A17-17R-RAySA$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón
000130774 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130774 590__ $$a2.145$$b2020
000130774 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b23 / 63 = 0.365$$c2020$$dQ2$$eT2
000130774 591__ $$aVETERINARY SCIENCES$$b43 / 146 = 0.295$$c2020$$dQ2$$eT1
000130774 591__ $$aREPRODUCTIVE BIOLOGY$$b26 / 30 = 0.867$$c2020$$dQ4$$eT3
000130774 592__ $$a0.572$$b2020
000130774 593__ $$aAnimal Science and Zoology$$c2020$$dQ2
000130774 593__ $$aMedicine (miscellaneous)$$c2020$$dQ2
000130774 593__ $$aFood Animals$$c2020$$dQ2
000130774 593__ $$aEndocrinology$$c2020$$dQ2
000130774 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130774 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, N.$$uUniversidad de Zaragoza
000130774 700__ $$0(orcid)0000-0002-1659-3387$$aMartínez, F.$$uUniversidad de Zaragoza
000130774 700__ $$aRevert, A.
000130774 700__ $$aMorrell, J.M.
000130774 700__ $$0(orcid)0000-0002-0797-1841$$aGil, L.$$uUniversidad de Zaragoza
000130774 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000130774 773__ $$g219, 106539 (2020), [7 pp]$$pAnim. reprod. sci.$$tAnimal Reproduction Science$$x0378-4320
000130774 8564_ $$s391434$$uhttps://zaguan.unizar.es/record/130774/files/texto_completo.pdf$$yPostprint
000130774 8564_ $$s553127$$uhttps://zaguan.unizar.es/record/130774/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000130774 909CO $$ooai:zaguan.unizar.es:130774$$particulos$$pdriver
000130774 951__ $$a2024-02-02-14:57:53
000130774 980__ $$aARTICLE