130774 20240202150202.0 doi 10.1016/j.anireprosci.2020.106539 sideral 118989 ART-2020-118989 eng Luño, V. Universidad de Zaragoza (orcid)0000-0002-7857-9732 Colloid centrifugation reduces bacterial load in chilled dog semen 2020 Access copy available to the general public Unrestricted Conventional semen extenders contain antibiotics to prevent bacterial growth. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage. Twenty-four ejaculates were obtained from healthy dogs by digital manipulation. Samples were diluted in Tris-citrate-fructose extender without antibiotics and divided into two treatment groups: SLC-selected samples and unselected samples. Sperm motility (CASA), viability and acrosome integrity (PI/FITC-PNA) as well as bacterial load of each microorganism species (colony-forming units/mL) were assessed at 0 and 48 h of storage at 4 °C. Results indicate SLC-selected dog spermatozoa have greater percentages of motility, viability and acrosome integrity (P < 0.05). Bacterial growth in SLC sperm samples was less (P < 0.05) than unselected samples. Removal of individual bacterial species varied from 91 % to 98 % for Escherichia coli (91.62 %), Streptococcus spp. (98.18 %), Staphylococcus spp.(95.33 %) and Pseudomonas spp. (92.50 %). In conclusion, the use of SLC with Canicoll has the potential to decrease bacterial load in chilled dog semen. info:eu-repo/grantAgreement/ES/DGA-FEDER/A17-17R-RAySA info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.145 2020 AGRICULTURE, DAIRY & ANIMAL SCIENCE 23 / 63 = 0.365 2020 Q2 T2 VETERINARY SCIENCES 43 / 146 = 0.295 2020 Q2 T1 REPRODUCTIVE BIOLOGY 26 / 30 = 0.867 2020 Q4 T3 0.572 2020 Animal Science and Zoology 2020 Q2 Medicine (miscellaneous) 2020 Q2 Food Animals 2020 Q2 Endocrinology 2020 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion González, N. Universidad de Zaragoza (orcid)0000-0002-1611-6337 Martínez, F. Universidad de Zaragoza (orcid)0000-0002-1659-3387 Revert, A. Morrell, J.M. Gil, L. Universidad de Zaragoza (orcid)0000-0002-0797-1841 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 219, 106539 (2020), [7 pp] Anim. reprod. sci. Animal Reproduction Science 0378-4320 391434 http://zaguan.unizar.es/record/130774/files/texto_completo.pdf Postprint 553127 http://zaguan.unizar.es/record/130774/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:130774 articulos driver 2024-02-02-14:57:53 ARTICLE