130782 20240319080945.0 doi 10.1080/10495398.2020.1811714 sideral 119016 ART-2022-119016 eng Luño, Victoria Universidad de Zaragoza (orcid)0000-0002-7857-9732 Specific phosphodiesterase type-10 inhibitor, papaverine, added after the cooling period improves canine sperm quality 2022 Access copy available to the general public Unrestricted The use of chilled semen has gained increasing interest in canine reproductive services. The addition of phosphodiesterase (PDE) inhibitors that increase the intracellular cyclic adenosine monophosphate levels may improve sperm motility. The purpose of this study was to examine the quality of sperm under the effect of the specific PDE-10 inhibitor (papaverine) added after storage for 1, 2, and 3 days at 5 °C. The ejaculates were obtained from 5 healthy Beagle dogs by digital manipulation. After collection, ejaculates were pooled, extended and cooled at 5 °C during 3 days. Sperm parameters were tested 30 min after the addition of different papaverine (PA) concentrations: 0, 5, 10 and 20 µM. Sperm motility (CASA), viability (PI/FITC-PNA) and capacitation status (chlortetracycline assay) were evaluated. The results showed that the addition of PA has no effect on sperm samples at day 0. However, concentrations of 5 and 10 µM increased (p < .05) sperm motility kinetics and viability significantly compared to the control at day 1, day 2 and day 3 of cooling. The addition of 20 μM PA decreased (p < .05) sperm quality parameters significantly and increased the percentage of capacitated/acrosome-reacted spermatozoa. In conclusion, the addition of 5 and 10 μM PA concentrations after cooled storage improved canine sperm quality. info:eu-repo/grantAgreement/ES/DGA-FEDER/A17-17R-RAySA info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.7 2022 AGRICULTURE, DAIRY & ANIMAL SCIENCE 6 / 62 = 0.097 2022 Q1 T1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 65 / 158 = 0.411 2022 Q2 T2 0.367 2022 Animal Science and Zoology 2022 Q2 Biotechnology 2022 Q3 Bioengineering 2022 Q3 3.3 2022 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion González, Noelia Universidad de Zaragoza (orcid)0000-0002-1611-6337 Martínez, Felisa Universidad de Zaragoza (orcid)0000-0002-1659-3387 González, Beatriz Olaciregui, Maite (orcid)0000-0001-6479-3933 Gil, Lydia Universidad de Zaragoza (orcid)0000-0002-0797-1841 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 33, 3 (2022), 519-525 Anim. biotechnol. ANIMAL BIOTECHNOLOGY 1049-5398 381016 http://zaguan.unizar.es/record/130782/files/texto_completo.pdf Postprint 556446 http://zaguan.unizar.es/record/130782/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:130782 articulos driver 2024-03-18-12:32:32 ARTICLE