130788 20241125101123.0 doi 10.1016/j.microc.2023.109403 sideral 136693 ART-2023-136693 eng Civera, Alba Universidad de Zaragoza (orcid)0000-0001-7719-6388 First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6 2023 Access copy available to the general public Unrestricted A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng mL−1 of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg g−1 of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique info:eu-repo/grantAgreement/ES/DGA/A20-23R info:eu-repo/grantAgreement/ES/MICINN/PID2019-103899RB-100 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.9 2023 CHEMISTRY, ANALYTICAL 19 / 106 = 0.179 2023 Q1 T1 0.742 2023 Analytical Chemistry 2023 Q1 Spectroscopy 2023 Q2 8.7 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Tvorynska, Sofiia Gamella, Maria Torrente-Rodríguez, Rebeca M. Pedrero, María Galán-Malo, Patricia Mata, Luis Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Barek, Jirí Pingarrón, José M. Campuzano, Susana Pérez, María D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 195 (2023), 109403 Microchem. j. MICROCHEMICAL JOURNAL 0026-265X 1567187 http://zaguan.unizar.es/record/130788/files/texto_completo.pdf Versión publicada 2562865 http://zaguan.unizar.es/record/130788/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:130788 articulos driver 2024-11-22-11:56:59 ARTICLE