000130925 001__ 130925 000130925 005__ 20250203110524.0 000130925 0247_ $$2doi$$a10.1007/s00232-015-9796-7 000130925 0248_ $$2sideral$$a92029 000130925 037__ $$aART-2015-92029 000130925 041__ $$aeng 000130925 100__ $$0(orcid)0000-0002-5490-3542$$aLuengo, E.$$uUniversidad de Zaragoza 000130925 245__ $$aA comparative study on the effects of millisecond- and microsecond-pulsed electric field treatments on the permeabilization and extraction of pigments from chlorella vulgaris 000130925 260__ $$c2015 000130925 5203_ $$aThe interdependencies of the two main processing parameters affecting “electroporation” (electric field strength and pulse duration) while using pulse duration in the range of milliseconds and microseconds on the permeabilization, inactivation, and extraction of pigments from Chlorella vulgaris was compared. While irreversible “electroporation” was observed above 4 kV/cm in the millisecond range, electric field strengths of =10 kV/cm were required in the microseconds range. However, to cause the electroporation of most of the 90 % of the population of C. vulgaris in the millisecond (5 kV/cm, 20 pulses) or microsecond (15 kV/cm, 25 pulses) range, the specific energy that was delivered was lower for microsecond treatments (16.87 kJ/L) than in millisecond treatments (150 kJ/L). In terms of the specific energy required to cause microalgae inactivation, treatments in the microsecond range also resulted in greater energy efficiency. The comparison of extraction yields in the range of milliseconds (5 kV, 20 ms) and microseconds (20, 25 pulses) under the conditions in which the maximum extraction was observed revealed that the improvement in the carotenoid extraction was similar and chlorophyll a and b extraction was slightly higher for treatments in the microsecond range. The specific energy that was required for the treatment in the millisecond range (150 kJ/L) was much higher than those required in the microsecond range (30 kJ/L). The comparison of the efficacy of both types of pulses on the extraction enhancement just after the treatment and after a post-pulse incubation period seemed to indicate that PEF in the millisecond range created irreversible alterations while, in the microsecond range, the defects were a dynamic structure along the post-pulse time that caused a subsequent increment in the extraction yield. 000130925 536__ $$9info:eu-repo/grantAgreement/EUR/COST/TD1104$$9info:eu-repo/grantAgreement/ES/DGA/A20 000130925 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000130925 590__ $$a1.991$$b2015 000130925 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b207 / 289 = 0.716$$c2015$$dQ3$$eT3 000130925 591__ $$aPHYSIOLOGY$$b49 / 83 = 0.59$$c2015$$dQ3$$eT2 000130925 591__ $$aCELL BIOLOGY$$b142 / 187 = 0.759$$c2015$$dQ4$$eT3 000130925 592__ $$a0.73$$b2015 000130925 593__ $$aBiophysics$$c2015$$dQ2 000130925 593__ $$aPhysiology$$c2015$$dQ3 000130925 593__ $$aCell Biology$$c2015$$dQ3 000130925 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion 000130925 700__ $$0(orcid)0000-0002-9337-417X$$aMartínez, J.M.$$uUniversidad de Zaragoza 000130925 700__ $$aCoustets, M. 000130925 700__ $$0(orcid)0000-0003-2430-858X$$aÁlvarez, I.$$uUniversidad de Zaragoza 000130925 700__ $$aTeissié, J. 000130925 700__ $$aRols, M.-P 000130925 700__ $$0(orcid)0000-0003-3957-9091$$aRaso, J.$$uUniversidad de Zaragoza 000130925 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos 000130925 773__ $$g248, 5 (2015), 883-891$$pJ. membr. biol.$$tJOURNAL OF MEMBRANE BIOLOGY$$x0022-2631 000130925 8564_ $$s707371$$uhttps://zaguan.unizar.es/record/130925/files/texto_completo.pdf$$yPostprint 000130925 8564_ $$s2424281$$uhttps://zaguan.unizar.es/record/130925/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint 000130925 909CO $$ooai:zaguan.unizar.es:130925$$particulos$$pdriver 000130925 951__ $$a2025-02-03-11:02:10 000130925 980__ $$aARTICLE