000131001 001__ 131001
000131001 005__ 20240202151704.0
000131001 0247_ $$2doi$$a10.1007/s00425-013-1861-9
000131001 0248_ $$2sideral$$a132286
000131001 037__ $$aART-2013-132286
000131001 041__ $$aeng
000131001 100__ $$0(orcid)0000-0002-4117-0159$$aAlonso-Simón, Ana
000131001 245__ $$aPurification and characterization of a soluble ß-1,4-glucan from bean (Phaseolus vulgaris L.)-cultured cells dehabituated to dichlobenil
000131001 260__ $$c2013
000131001 5203_ $$aBean cells habituated to grow in the presence of dichlobenil exhibited reduced cellulose and hemicellulose content and an increase in pectic polysaccharides. Furthermore, following the extraction of pectins and hemicelluloses, a large amount of neutral sugars was released. These sugars were found to be part of a soluble β-1,4-glucan in a preliminary characterization, as reported by Encina et al. (Physiol Plant 114:182–191, 2002). When habituated cells were subcultured in the absence of the herbicide (dehabituated cells), the release of neutral sugars after the extraction of pectins and hemicelluloses was maintained. In this study, we have isolated a soluble β-1,4-glucan from dehabituated cells by sonication of the wall residue (cellulose fraction) remaining after fractionation. Gel filtration chromatography revealed that its average molecular size was 14 kDa. Digestion of the sample with endocellulase revealed the presence of cellobiose, cellotriose, and cellotetraose. Methylation analysis showed that 4-linked glucose was the most abundant sugar residue, but 4,6-linked glucose, terminal arabinose and 4-linked galactose for xyloglucan, and arabinogalactan were also identified. NMR analysis showed that this 1,4-glucan may be composed of various kinds of substitutions along the glucan backbone together with acetyl groups linked to the OH group of sugar residues. Thus, despite its relatively high molecular mass, the β-glucan remains soluble because of its unique configuration. This is the first time that a glucan with such characteristics has been isolated and described. The discovery of new molecules, as this β-glucan with unique features, may help understand the composition and arrangement of the polymers within plant cell walls, contributing to a better understanding of this complex structure.
000131001 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000131001 590__ $$a3.376$$b2013
000131001 591__ $$aPLANT SCIENCES$$b26 / 197 = 0.132$$c2013$$dQ1$$eT1
000131001 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000131001 700__ $$aEncina, Antonio E.
000131001 700__ $$aSeyama, Tomoko
000131001 700__ $$aKondo, Tetsuo
000131001 700__ $$aGarcía-Angulo, Penélope
000131001 700__ $$aÁlvarez, Jesús M.
000131001 700__ $$aAcebes, Jose L.
000131001 700__ $$aHayashi, Takahisa
000131001 773__ $$g237 (2013), 1475-1482$$pPlanta$$tPLANTA$$x0032-0935
000131001 8564_ $$s336945$$uhttps://zaguan.unizar.es/record/131001/files/texto_completo.pdf$$yVersión publicada
000131001 8564_ $$s2169753$$uhttps://zaguan.unizar.es/record/131001/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000131001 909CO $$ooai:zaguan.unizar.es:131001$$particulos$$pdriver
000131001 951__ $$a2024-02-02-14:52:32
000131001 980__ $$aARTICLE