000131040 001__ 131040
000131040 005__ 20240208144955.0
000131040 0247_ $$2doi$$a10.1007/s00232-014-9688-2
000131040 0248_ $$2sideral$$a85738
000131040 037__ $$aART-2014-85738
000131040 041__ $$aeng
000131040 100__ $$0(orcid)0000-0002-5490-3542$$aLuengo, Elisa$$uUniversidad de Zaragoza
000131040 245__ $$aEffect of Pulsed Electric Field Treatments on Permeabilization and Extraction of Pigments from Chlorella vulgaris
000131040 260__ $$c2014
000131040 5060_ $$aAccess copy available to the general public$$fUnrestricted
000131040 5203_ $$aThe effect of pulsed electric field (PEF) treatments of different intensities on the electroporation of the cytoplasmatic membrane of Chlorella vulgaris, and on the extraction of carotenoids and chlorophylls were investigated. Staining the cells with propidium iodide before and after the PEF treatment revealed the existence of reversible and irreversible electroporation. Application of PEF treatments in the range of 20–25 kV cm-1 caused most of the population of C. vulgaris to be irreversibly electroporated even at short treatment times (5 pulses of 3 µs). However, at lower electric field strengths (10 kV cm-1), cells that were reversibly electroporated were observed even after 50 pulses of 3 µs. The electroporation of C. vulgaris cells by PEF higher than 15 kV cm-1 and duration is higher than 15 µs increased significantly the extraction yield of intracellular components of C. vulgaris. The application of a 20 kV cm-1 for 75 µs increased the extraction yield just after the PEF treatment of the carotenoids, and chlorophylls a and b 0.5, 0.7, and 0.8 times, respectively. However, further increments in electric field strength and treatment time did not cause significant increments in the extraction yield. The extraction of carotenoids from PEF-treated C. vulgaris cells after 1 h of the application of the treatment significantly increased the extraction yield in comparison to the yield obtained from the cells extracted just after the PEF treatment. After PEF treatment at 20 kV cm-1 for 75 µs, extraction yield for carotenoids, and chlorophylls a and b increased 1.2, 1.6, and 2.1 times, respectively. A high correlation was observed between irreversible electroporation and percentage of yield increase when the extraction was conducted after 1 h of the application of PEF treatment (R: 0.93), but not when the extraction was conducted just after PEF treatment (R: 0.67).
000131040 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000131040 590__ $$a2.457$$b2014
000131040 591__ $$aPHYSIOLOGY$$b37 / 83 = 0.446$$c2014$$dQ2$$eT2
000131040 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b166 / 290 = 0.572$$c2014$$dQ3$$eT2
000131040 591__ $$aCELL BIOLOGY$$b122 / 184 = 0.663$$c2014$$dQ3$$eT3
000131040 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000131040 700__ $$0(orcid)0000-0002-2042-9598$$aCondón-Abanto, Santiago$$uUniversidad de Zaragoza
000131040 700__ $$0(orcid)0000-0003-2430-858X$$aÁlvarez, Ignacio$$uUniversidad de Zaragoza
000131040 700__ $$0(orcid)0000-0003-3957-9091$$aRaso, Javier$$uUniversidad de Zaragoza
000131040 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000131040 773__ $$g247, 12 (2014), 1269-1277$$pJ. membr. biol.$$tJOURNAL OF MEMBRANE BIOLOGY$$x0022-2631
000131040 8564_ $$s486815$$uhttps://zaguan.unizar.es/record/131040/files/texto_completo.pdf$$yPostprint
000131040 8564_ $$s583239$$uhttps://zaguan.unizar.es/record/131040/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000131040 909CO $$ooai:zaguan.unizar.es:131040$$particulos$$pdriver
000131040 951__ $$a2024-02-08-14:47:06
000131040 980__ $$aARTICLE