000131045 001__ 131045 000131045 005__ 20240202151704.0 000131045 0247_ $$2doi$$a10.3390/ijms25031510 000131045 0248_ $$2sideral$$a136678 000131045 037__ $$aART-2024-136678 000131045 041__ $$aeng 000131045 100__ $$aMalo, Clara$$uUniversidad de Zaragoza 000131045 245__ $$aIn vitro growth of human follicles: current and Future Perspectives 000131045 260__ $$c2024 000131045 5060_ $$aAccess copy available to the general public$$fUnrestricted 000131045 5203_ $$aOvarian tissue cryopreservation is gaining importance as a successful method to restore fertility to girls and young women at high risk of sterility. However, there are concerns regarding the safety of transplantation after ovarian tissue cryopreservation due to the high risk of reintroducing cancer cells and causing disease recurrence. In these cases, the development of culture systems that support oocyte development from the primordial follicle stage is required. Notable achievements have been reached in human follicle in vitro growth in the past decade. Currently, systems for the in vitro culture of ovarian tissue are based on two-dimensional substrates that do not support the survival of follicles or recapitulate the mechanical heterogenicity in the mammalian ovary. Recognition of the importance of special arrangements between cells has spurred research in three-dimensional culture systems, and the provision of a precise culture system that maximizes the diffusion of nutrients and gases through the follicles has raised interest in advanced biomimetic models. The current review critically examines various culture systems employed for the in vitro development of follicles, with a particular focus on solutions utilizing Organ-on-a-Chip (OOC) technology. The emphasis on OOC technology underscores its role as a promising avenue in ensuring the successful cultivation and maintenance of follicular structures during the culture period. 000131045 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000131045 655_4 $$ainfo:eu-repo/semantics/review$$vinfo:eu-repo/semantics/publishedVersion 000131045 700__ $$0(orcid)0000-0003-0156-4230$$aOliván, Sara$$uUniversidad de Zaragoza 000131045 700__ $$0(orcid)0000-0003-2410-5678$$aOchoa, Ignacio$$uUniversidad de Zaragoza 000131045 700__ $$aShikanov, Ariella 000131045 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología 000131045 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000131045 773__ $$g25, 3 (2024), 1510 [13 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596 000131045 8564_ $$s1887841$$uhttps://zaguan.unizar.es/record/131045/files/texto_completo.pdf$$yVersión publicada 000131045 8564_ $$s2679042$$uhttps://zaguan.unizar.es/record/131045/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000131045 909CO $$ooai:zaguan.unizar.es:131045$$particulos$$pdriver 000131045 951__ $$a2024-02-02-15:07:31 000131045 980__ $$aARTICLE