131045 20260217205510.0 doi 10.3390/ijms25031510 sideral 136678 ART-2024-136678 eng Malo, Clara Universidad de Zaragoza In vitro growth of human follicles: current and Future Perspectives 2024 Access copy available to the general public Unrestricted Ovarian tissue cryopreservation is gaining importance as a successful method to restore fertility to girls and young women at high risk of sterility. However, there are concerns regarding the safety of transplantation after ovarian tissue cryopreservation due to the high risk of reintroducing cancer cells and causing disease recurrence. In these cases, the development of culture systems that support oocyte development from the primordial follicle stage is required. Notable achievements have been reached in human follicle in vitro growth in the past decade. Currently, systems for the in vitro culture of ovarian tissue are based on two-dimensional substrates that do not support the survival of follicles or recapitulate the mechanical heterogenicity in the mammalian ovary. Recognition of the importance of special arrangements between cells has spurred research in three-dimensional culture systems, and the provision of a precise culture system that maximizes the diffusion of nutrients and gases through the follicles has raised interest in advanced biomimetic models. The current review critically examines various culture systems employed for the in vitro development of follicles, with a particular focus on solutions utilizing Organ-on-a-Chip (OOC) technology. The emphasis on OOC technology underscores its role as a promising avenue in ensuring the successful cultivation and maintenance of follicular structures during the culture period. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 4.9 2024 BIOCHEMISTRY & MOLECULAR BIOLOGY 72 / 320 = 0.225 2024 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 70 / 239 = 0.293 2024 Q2 T1 1.273 2024 Medicine (miscellaneous) 2024 Q1 Physical and Theoretical Chemistry 2024 Q1 Computer Science Applications 2024 Q1 Inorganic Chemistry 2024 Q1 Spectroscopy 2024 Q1 Organic Chemistry 2024 Q1 Molecular Biology 2024 Q2 Catalysis 2024 Q2 9.0 2024 info:eu-repo/semantics/review info:eu-repo/semantics/publishedVersion Oliván, Sara Universidad de Zaragoza (orcid)0000-0003-0156-4230 Ochoa, Ignacio Universidad de Zaragoza (orcid)0000-0003-2410-5678 Shikanov, Ariella 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 25, 3 (2024), 1510 [13 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 1887841 http://zaguan.unizar.es/record/131045/files/texto_completo.pdf Versión publicada 2679042 http://zaguan.unizar.es/record/131045/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131045 articulos driver 2026-02-17-20:24:51 ARTICLE