131188 20251219150008.0 doi 10.1016/j.foodchem.2020.126745 sideral 117714 ART-2020-117714 eng Tobajas, Ana P. Universidad de Zaragoza (orcid)0000-0002-2646-5733 Effect of high pressure and pulsed electric field on denaturation and allergenicity of Pru p 3 protein from peach 2020 Access copy available to the general public Unrestricted The effect of high pressure (HP) and pulsed electric field (PEF) treatments combined or not with heat on denaturation and allergenicity of Pru p 3, the major allergenic protein of peach, was studied. Denaturation of Pru p 3, determined by ELISA using rabbit IgG, occurred when the protein was treated at 500 and 600 MPa at 20 °C and at 400 MPa at 50 °C. PEF treatment at 25 kV/cm at 50 °C denatures Pru p 3. Allergenicity of Pru p 3 was estimated in vitro by a competitive fluorescent immunoassay using three pools of sera from peach allergic patients. Any treatment applied did not show to influence the binding of Pru p 3 to IgE. When HP and PEF treated samples were tested by the prick test, the skin response was dependent on the particular sensitization of each patient, obtaining an increased reaction in more than 50% of individuals. info:eu-repo/grantAgreement/ES/DGA-FEDER/A02-17R info:eu-repo/semantics/openAccess by-nc-nd https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es 7.514 2020 CHEMISTRY, APPLIED 7 / 74 = 0.095 2020 Q1 T1 NUTRITION & DIETETICS 6 / 87 = 0.069 2020 Q1 T1 FOOD SCIENCE & TECHNOLOGY 7 / 143 = 0.049 2020 Q1 T1 1.772 2020 Analytical Chemistry 2020 Q1 Medicine (miscellaneous) 2020 Q1 Food Science 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Agulló-García, Ana Cubero, José L. (orcid)0000-0002-5232-685X Colás, Carlos Universidad de Zaragoza (orcid)0000-0001-8959-6784 Segura-Gil, Isabel Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X Calvo, Miguel Universidad de Zaragoza (orcid)0000-0001-5373-9210 Pérez, María D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 321 (2020), 126745 1-8 Food chem. Food Chemistry 0308-8146 929010 http://zaguan.unizar.es/record/131188/files/texto_completo.pdf Postprint 2542187 http://zaguan.unizar.es/record/131188/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:131188 articulos driver 2025-12-19-14:58:09 ARTICLE