000131312 001__ 131312
000131312 005__ 20240319081006.0
000131312 0247_ $$2doi$$a10.1039/d2fo00287f
000131312 0248_ $$2sideral$$a129359
000131312 037__ $$aART-2022-129359
000131312 041__ $$aeng
000131312 100__ $$0(orcid)0000-0002-6546-6149$$aBelles, Andrea$$uUniversidad de Zaragoza
000131312 245__ $$aLactoferrin modulates gut microbiota and Toll-like receptors (TLRs) in mice with dysbiosis induced by antibiotics
000131312 260__ $$c2022
000131312 5060_ $$aAccess copy available to the general public$$fUnrestricted
000131312 5203_ $$aBackground: Antibiotic administration can result in gut microbiota and immune system alterations that impact health. Bovine lactoferrin is a milk protein with anticancer, anti-inflammatory, antimicrobial and immune modulatory activities. The aim was to study the ability of native and iron-saturated lactoferrin to reverse the effects of clindamycin on gut microbiota and intestinal Toll-like receptor (TLR) expression in a murine model. Methods: Male C57BL/6 mice were treated with vehicle, clindamycin (Clin), native bovine lactoferrin (nLf), nLf + clindamycin (nLf_Clin), iron-saturated bovine lactoferrin (sLf) and sLf + clindamycin (sLf_Clin). Fecal samples of each group were collected, and bacterial DNA was extracted. Sequencing of 16s rRNA V4 hypervariable gene regions was conducted to assess the microbial composition. mRNA expression levels of TLRs (1-9) were determined in mouse colon by qPCR. Pearson''s correlation test was carried out between bacteria showing differences in abundance among samples and TLR2, TLR8 and TLR9. Results: Beta-diversity analysis showed that the microbial community of the vehicle was different from the communities of Clin, nLf_Clin and sLf_Clin. At the family level, Bacteroidaceae, Prevotellaceae and Rikenellaceae decreased in the Clin group, and treatment with nLf or sLf reverted these effects. Clin reduced the expression of TLR2, TLR8 and TLR9 and sLf reverted the decrease in the expression of these receptors. Finally, TLR8 was positively correlated with Rikenellaceae abundance. Conclusion: In a situation of intestinal dysbiosis induced by clindamycin, lactoferrin restores the normal levels of some anti-inflammatory bacteria and TLRs and, therefore, could be a good ingredient to be added to functional foods.
000131312 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A20-20R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987
000131312 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000131312 590__ $$a6.1$$b2022
000131312 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b22 / 142 = 0.155$$c2022$$dQ1$$eT1
000131312 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b59 / 285 = 0.207$$c2022$$dQ1$$eT1
000131312 592__ $$a1.103$$b2022
000131312 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000131312 593__ $$aFood Science$$c2022$$dQ1
000131312 594__ $$a9.6$$b2022
000131312 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000131312 700__ $$0(orcid)0000-0002-1829-6319$$aAguirre-Ramirez, Diego$$uUniversidad de Zaragoza
000131312 700__ $$aAbad, Inés$$uUniversidad de Zaragoza
000131312 700__ $$aParras-Molto, Marcos
000131312 700__ $$0(orcid)0000-0001-5964-823X$$aSánchez, Lourdes$$uUniversidad de Zaragoza
000131312 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, Laura$$uUniversidad de Zaragoza
000131312 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000131312 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000131312 773__ $$g13 (2022), 5854-5869$$pFOOD & FUNCTION$$tFOOD & FUNCTION$$x2042-6496
000131312 8564_ $$s1706345$$uhttps://zaguan.unizar.es/record/131312/files/texto_completo.pdf$$yPostprint
000131312 8564_ $$s2717836$$uhttps://zaguan.unizar.es/record/131312/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000131312 909CO $$ooai:zaguan.unizar.es:131312$$particulos$$pdriver
000131312 951__ $$a2024-03-18-14:37:30
000131312 980__ $$aARTICLE