000131317 001__ 131317
000131317 005__ 20240207154753.0
000131317 0247_ $$2doi$$a10.1242/dev.113886
000131317 0248_ $$2sideral$$a136578
000131317 037__ $$aART-2015-136578
000131317 041__ $$aeng
000131317 100__ $$aAcosta, Helena
000131317 245__ $$aThe serpin PN1 is a feedback regulator of FGF signaling in germ layer and primary axis formation
000131317 260__ $$c2015
000131317 5060_ $$aAccess copy available to the general public$$fUnrestricted
000131317 5203_ $$aGerm layer formation and primary axis development rely on Fibroblast growth factors (FGFs). In Xenopus, the secreted serine protease HtrA1 induces mesoderm and posterior trunk/tail structures by facilitating the spread of FGF signals. Here, we show that the serpin Protease nexin-1 (PN1) is transcriptionally activated by FGF signals, suppresses mesoderm and promotes head development in mRNA-injected embryos. An antisense morpholino oligonucleotide against PN1 has the opposite effect and inhibits ectodermal fate. However, ectoderm and anterior head structures can be restored in PN1-depleted embryos when HtrA1 and FGF receptor activities are diminished, indicating that FGF signals negatively regulate their formation. We show that PN1 binds to and inhibits HtrA1, prevents degradation of the proteoglycan Syndecan 4 and restricts paracrine FGF/Erk signaling. Our data suggest that PN1 is a negative-feedback regulator of FGF signaling and has important roles in ectoderm and head development.
000131317 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000131317 590__ $$a6.059$$b2015
000131317 591__ $$aDEVELOPMENTAL BIOLOGY$$b4 / 41 = 0.098$$c2015$$dQ1$$eT1
000131317 592__ $$a5.235$$b2015
000131317 593__ $$aMolecular Biology$$c2015$$dQ1
000131317 593__ $$aDevelopmental Biology$$c2015$$dQ1
000131317 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000131317 700__ $$aIliev, Dobromir
000131317 700__ $$aGrahn, Tan Hooi Min
000131317 700__ $$aGouignard, Nadège
000131317 700__ $$aMaccarana, Marco
000131317 700__ $$aGriesbach, Julia
000131317 700__ $$aHerzmann, Svende
000131317 700__ $$aSagha, Mohsen
000131317 700__ $$0(orcid)0000-0002-0606-0763$$aCliment, Maria$$uUniversidad de Zaragoza
000131317 700__ $$aPera, Edgar M.
000131317 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000131317 773__ $$g142, 6 (2015), 1146-1158$$pDevelopment$$tDevelopment (Cambridge, England)$$x1477-9129
000131317 787__ $$tThe serpin PN1 is a feedback regulator of FGF signaling in germ layer and primary axis formation$$w10.1242/dev.113886
000131317 8564_ $$s2114630$$uhttps://zaguan.unizar.es/record/131317/files/texto_completo.pdf$$yVersión publicada
000131317 8564_ $$s3541119$$uhttps://zaguan.unizar.es/record/131317/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000131317 909CO $$ooai:zaguan.unizar.es:131317$$particulos$$pdriver
000131317 951__ $$a2024-02-07-14:40:15
000131317 980__ $$aARTICLE