131317 20240207154753.0 doi 10.1242/dev.113886 sideral 136578 ART-2015-136578 eng Acosta, Helena The serpin PN1 is a feedback regulator of FGF signaling in germ layer and primary axis formation 2015 Germ layer formation and primary axis development rely on Fibroblast growth factors (FGFs). In Xenopus, the secreted serine protease HtrA1 induces mesoderm and posterior trunk/tail structures by facilitating the spread of FGF signals. Here, we show that the serpin Protease nexin-1 (PN1) is transcriptionally activated by FGF signals, suppresses mesoderm and promotes head development in mRNA-injected embryos. An antisense morpholino oligonucleotide against PN1 has the opposite effect and inhibits ectodermal fate. However, ectoderm and anterior head structures can be restored in PN1-depleted embryos when HtrA1 and FGF receptor activities are diminished, indicating that FGF signals negatively regulate their formation. We show that PN1 binds to and inhibits HtrA1, prevents degradation of the proteoglycan Syndecan 4 and restricts paracrine FGF/Erk signaling. Our data suggest that PN1 is a negative-feedback regulator of FGF signaling and has important roles in ectoderm and head development. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 6.059 2015 DEVELOPMENTAL BIOLOGY 4 / 41 = 0.098 2015 Q1 T1 5.235 2015 Molecular Biology 2015 Q1 Developmental Biology 2015 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Iliev, Dobromir Grahn, Tan Hooi Min Gouignard, Nadège Maccarana, Marco Griesbach, Julia Herzmann, Svende Sagha, Mohsen Climent, Maria Universidad de Zaragoza (orcid)0000-0002-0606-0763 Pera, Edgar M. 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 142, 6 (2015), 1146-1158 Development Development (Cambridge, England) 1477-9129 The serpin PN1 is a feedback regulator of FGF signaling in germ layer and primary axis formation 10.1242/dev.113886 2114630 http://zaguan.unizar.es/record/131317/files/texto_completo.pdf Versión publicada 3541119 http://zaguan.unizar.es/record/131317/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131317 articulos driver 2024-02-07-14:40:15 ARTICLE