000131334 001__ 131334 000131334 005__ 20240207154753.0 000131334 0247_ $$2doi$$a10.1645/0022-3395(2001)087[0946:ETHEDS]2.0.CO;2 000131334 0248_ $$2sideral$$a51155 000131334 037__ $$aART-2001-51155 000131334 041__ $$aeng 000131334 100__ $$0(orcid)0000-0002-9453-7655$$aDel Cacho, E$$uUniversidad de Zaragoza 000131334 245__ $$aEimeria tenella: Hsp70 expression during sporogony 000131334 260__ $$c2001 000131334 5203_ $$aThere is an increasing interest in identifying the parasite components involved in the maturation, development, and infectivity of intracellular protozoan parasites. In the present study, a heat shock protein (hsp) of the family of 70 kDa hsp (hsp70), which play important roles in the stage conversion and virulence of these parasites, was examined. Whereas hsp70 expression has been examined in Eimeria tenella within host tissues, in the present study, oocysts of E. tenella were used to investigate the expression of hsp70 during sporulation without interference from the host; hsp70 expression during excystation was induced by incubating sporulated oocysts under various experimental conditions to produce the stimuli necessary for sporozoites to become active and to excyst in vitro. Hsp70 was detected by immunohistochemical techniques; quantitative flow cytometric analysis was also been carried out using specific monoclonal antibodies against hsp70. Hsp70 was expressed during sporulation but was not found in sporulated oocysts after the completion of sporulation. Oocysts re-expressed hsp70 when excystation was induced. The presence of hsp70 prior to infection may preadapt the parasite for additional stress in the host and may be involved in the formation of sporozoites. 000131334 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000131334 590__ $$a1.521$$b2001 000131334 591__ $$aPARASITOLOGY$$b7 / 21 = 0.333$$c2001$$dQ2$$eT2 000131334 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000131334 700__ $$0(orcid)0000-0003-3945-4854$$aGallego, M$$uUniversidad de Zaragoza 000131334 700__ $$aPereboom, D 000131334 700__ $$aLópez Bernad, F 000131334 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, J$$uUniversidad de Zaragoza 000131334 700__ $$0(orcid)0000-0002-3532-0384$$aSánchez Acedo, C.$$uUniversidad de Zaragoza 000131334 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000131334 773__ $$g87, 5 (2001), 946-950$$pJ. parasitol.$$tJOURNAL OF PARASITOLOGY$$x0022-3395 000131334 8564_ $$s246032$$uhttps://zaguan.unizar.es/record/131334/files/texto_completo.pdf$$yVersión publicada 000131334 8564_ $$s3828990$$uhttps://zaguan.unizar.es/record/131334/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000131334 909CO $$ooai:zaguan.unizar.es:131334$$particulos$$pdriver 000131334 951__ $$a2024-02-07-14:41:09 000131334 980__ $$aARTICLE