doi:10.1556/004.2020.00013engSevilla, E.Mainar-Jaime, R.C.Moreno, B.Martin-Burriel, I.Morales, M.Andres-Lasheras, S.Chirino-Trejo, M.Badiola, J.J.Bolea, R.Antimicrobial resistance among canine enteric Escherichia coli isolates and prevalence of attaching-effacing and extraintestinal pathogenic virulence factors in SpainART-2020-118748The aim of this study was to estimate the prevalence of antimicrobial resistance (AMR) in Escherichia coli from a dog population in Spain and assess specific virulence factors. Susceptibility to 22 antimicrobials was tested along with the production of extended-spectrum beta-lactamases (ESBLs) and AmpC in faecal isolates from 100 dogs. Virulence-related genes associated with attaching and effacing E. coli (eae, Stx1, Stx2) and extraintestinal pathogenic E. coli - ExPEC - (papC, hlyA and cnf1) were detected by PCR. At least one kind of AMR was observed in 73% of the isolates. The highest prevalences corresponded to penicillin (45%), aminoglycoside (40%) and non-extended spectrum cephalosporin (39%) classes. Multidrug resistance (MDR) was observed in 53.4% of the resistant isolates. No resistance to colistin was found. Production of ESBL/AmpC enzymes was detected in 5% of E. coli. Shiga toxin-producing E. coli were not observed, enteropathogenic E. coli were identified in only 12% of them, and ExPEC were found in 25%. Dog faeces can be a source of E. coli strains potentially presenting a threat to humans through their virulence factors or AMR. The non-hygienic keeping of animals may increase the risk of colonisation of such pathogens in humans.2020http://zaguan.unizar.es/record/13139510.1556/004.2020.00013http://zaguan.unizar.es/record/131395oai:zaguan.unizar.es:131395info:eu-repo/grantAgreement/ES/DGA/A05-17Rinfo:eu-repo/grantAgreement/ES/DGA/A13-17Rinfo:eu-repo/grantAgreement/ES/MICIU/FPU14-02035ACTA VETERINARIA HUNGARICA 68, 1 (2020), 1-7byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess