131395 20240209144725.0 doi 10.1556/004.2020.00013 sideral 118748 ART-2020-118748 eng Sevilla, E. (orcid)0000-0002-4213-2904 Antimicrobial resistance among canine enteric Escherichia coli isolates and prevalence of attaching-effacing and extraintestinal pathogenic virulence factors in Spain 2020 Access copy available to the general public Unrestricted The aim of this study was to estimate the prevalence of antimicrobial resistance (AMR) in Escherichia coli from a dog population in Spain and assess specific virulence factors. Susceptibility to 22 antimicrobials was tested along with the production of extended-spectrum beta-lactamases (ESBLs) and AmpC in faecal isolates from 100 dogs. Virulence-related genes associated with attaching and effacing E. coli (eae, Stx1, Stx2) and extraintestinal pathogenic E. coli - ExPEC - (papC, hlyA and cnf1) were detected by PCR. At least one kind of AMR was observed in 73% of the isolates. The highest prevalences corresponded to penicillin (45%), aminoglycoside (40%) and non-extended spectrum cephalosporin (39%) classes. Multidrug resistance (MDR) was observed in 53.4% of the resistant isolates. No resistance to colistin was found. Production of ESBL/AmpC enzymes was detected in 5% of E. coli. Shiga toxin-producing E. coli were not observed, enteropathogenic E. coli were identified in only 12% of them, and ExPEC were found in 25%. Dog faeces can be a source of E. coli strains potentially presenting a threat to humans through their virulence factors or AMR. The non-hygienic keeping of animals may increase the risk of colonisation of such pathogens in humans. info:eu-repo/grantAgreement/ES/DGA/A05-17R info:eu-repo/grantAgreement/ES/DGA/A13-17R info:eu-repo/grantAgreement/ES/MICIU/FPU14-02035 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 0.955 2020 VETERINARY SCIENCES 101 / 146 = 0.692 2020 Q3 T3 0.395 2020 Veterinary (miscellaneous) 2020 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Mainar-Jaime, R.C. Universidad de Zaragoza (orcid)0000-0001-5442-7702 Moreno, B. Universidad de Zaragoza (orcid)0000-0001-6629-6842 Martin-Burriel, I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Morales, M. Universidad de Zaragoza (orcid)0000-0001-9778-6545 Andres-Lasheras, S. Chirino-Trejo, M. Badiola, J.J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Bolea, R. Universidad de Zaragoza (orcid)0000-0002-2746-3932 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 68, 1 (2020), 1-7 Acta vet. Hung. ACTA VETERINARIA HUNGARICA 0236-6290 168241 http://zaguan.unizar.es/record/131395/files/texto_completo.pdf Postprint 1094354 http://zaguan.unizar.es/record/131395/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:131395 articulos driver 2024-02-09-14:44:51 ARTICLE