000131485 001__ 131485
000131485 005__ 20240209155915.0
000131485 0247_ $$2doi$$a10.1186/s13567-019-0718-z
000131485 0248_ $$2sideral$$a136916
000131485 037__ $$aART-2019-136916
000131485 041__ $$aeng
000131485 100__ $$aLangeveld, Jan P. M.
000131485 245__ $$aFour types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods
000131485 260__ $$c2019
000131485 5060_ $$aAccess copy available to the general public$$fUnrestricted
000131485 5203_ $$aScrapie in goats has been known since 1942, the archetype of prion diseases in which only prion protein (PrP) in misfolded state (PrPSc) acts as infectious agent with fatal consequence. Emergence of bovine spongiform encephalopathy (BSE) with its zoonotic behaviour and detection in goats enhanced fears that its source was located in small ruminants. However, in goats knowledge on prion strain typing is limited. A European-wide study is presented concerning the biochemical phenotypes of the protease resistant fraction of PrPSc (PrPres) in over thirty brain isolates from transmissible spongiform encephalopathy (TSE) affected goats collected in seven countries. Three different scrapie forms were found: classical scrapie (CS), Nor98/atypical scrapie and one case of CH1641 scrapie. In addition, CS was found in two variants—CS-1 and CS-2 (mainly Italy)—which differed in proteolytic resistance of the PrPresN-terminus. Suitable PrPres markers for discriminating CH1641 from BSE (C-type) appeared to be glycoprofile pattern, presence of two triplets instead of one, and structural (in)stability of its core amino acid region. None of the samples exhibited BSE like features. BSE and these four scrapie types, of which CS-2 is new, can be recognized in goats with combinations of a set of nine biochemical parameters.
000131485 536__ $$9info:eu-repo/grantAgreement/EC/FP6/36353/EU/Goat BSE: proposal for improvement of goat TSE discriminative diagnosis and susceptibility based assessment of BSE infectivity in goat milk and meat/GOATBSE$$9info:eu-repo/grantAgreement/EC/FP6/506579/EU/Prevention, control and management of prion diseases/NEUROPRION$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2012-40071
000131485 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000131485 590__ $$a3.357$$b2019
000131485 591__ $$aVETERINARY SCIENCES$$b6 / 141 = 0.043$$c2019$$dQ1$$eT1
000131485 592__ $$a1.425$$b2019
000131485 593__ $$aVeterinary (miscellaneous)$$c2019$$dQ1
000131485 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000131485 700__ $$aPirisinu, Laura
000131485 700__ $$aJacobs, Jorg G.
000131485 700__ $$aMazza, Maria
000131485 700__ $$aLantier, Isabelle
000131485 700__ $$aSimon, Stéphanie
000131485 700__ $$aAndréoletti, Olivier
000131485 700__ $$0(orcid)0000-0001-5105-6133$$aAcin, Cristina$$uUniversidad de Zaragoza
000131485 700__ $$aEsposito, Elena
000131485 700__ $$aFast, Christine
000131485 700__ $$aGroschup, Martin
000131485 700__ $$aGoldmann, Wilfred
000131485 700__ $$aSpiropoulos, John
000131485 700__ $$aSklaviadis, Theodoros
000131485 700__ $$aLantier, Frederic
000131485 700__ $$aEkateriniadou, Loukia
000131485 700__ $$aPapasavva-Stylianou, Penelope
000131485 700__ $$avan Keulen, Lucien J. M.
000131485 700__ $$aAcutis, Pier-Luigi
000131485 700__ $$aAgrimi, Umberto
000131485 700__ $$aBossers, Alex
000131485 700__ $$aNonno, Romolo
000131485 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000131485 773__ $$g50, 97 (2019), 1-13$$pVet. res.$$tVeterinary Research$$x0928-4249
000131485 8564_ $$s1741802$$uhttps://zaguan.unizar.es/record/131485/files/texto_completo.pdf$$yVersión publicada
000131485 8564_ $$s2645444$$uhttps://zaguan.unizar.es/record/131485/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000131485 909CO $$ooai:zaguan.unizar.es:131485$$particulos$$pdriver
000131485 951__ $$a2024-02-09-14:31:25
000131485 980__ $$aARTICLE