131485 20240209155915.0 doi 10.1186/s13567-019-0718-z sideral 136916 ART-2019-136916 eng Langeveld, Jan P. M. Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods 2019 Scrapie in goats has been known since 1942, the archetype of prion diseases in which only prion protein (PrP) in misfolded state (PrPSc) acts as infectious agent with fatal consequence. Emergence of bovine spongiform encephalopathy (BSE) with its zoonotic behaviour and detection in goats enhanced fears that its source was located in small ruminants. However, in goats knowledge on prion strain typing is limited. A European-wide study is presented concerning the biochemical phenotypes of the protease resistant fraction of PrPSc (PrPres) in over thirty brain isolates from transmissible spongiform encephalopathy (TSE) affected goats collected in seven countries. Three different scrapie forms were found: classical scrapie (CS), Nor98/atypical scrapie and one case of CH1641 scrapie. In addition, CS was found in two variants—CS-1 and CS-2 (mainly Italy)—which differed in proteolytic resistance of the PrPresN-terminus. Suitable PrPres markers for discriminating CH1641 from BSE (C-type) appeared to be glycoprofile pattern, presence of two triplets instead of one, and structural (in)stability of its core amino acid region. None of the samples exhibited BSE like features. BSE and these four scrapie types, of which CS-2 is new, can be recognized in goats with combinations of a set of nine biochemical parameters. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/EC/FP6/36353/EU/Goat BSE: proposal for improvement of goat TSE discriminative diagnosis and susceptibility based assessment of BSE infectivity in goat milk and meat/GOATBSE info:eu-repo/grantAgreement/EC/FP6/506579/EU/Prevention, control and management of prion diseases/NEUROPRION info:eu-repo/grantAgreement/ES/MINECO/AGL2012-40071 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.357 2019 VETERINARY SCIENCES 6 / 141 = 0.043 2019 Q1 T1 1.425 2019 Veterinary (miscellaneous) 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pirisinu, Laura Jacobs, Jorg G. Mazza, Maria Lantier, Isabelle Simon, Stéphanie Andréoletti, Olivier Acin, Cristina Universidad de Zaragoza (orcid)0000-0001-5105-6133 Esposito, Elena Fast, Christine Groschup, Martin Goldmann, Wilfred Spiropoulos, John Sklaviadis, Theodoros Lantier, Frederic Ekateriniadou, Loukia Papasavva-Stylianou, Penelope van Keulen, Lucien J. M. Acutis, Pier-Luigi Agrimi, Umberto Bossers, Alex Nonno, Romolo 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 50, 97 (2019), 1-13 Vet. res. Veterinary Research 0928-4249 1741802 http://zaguan.unizar.es/record/131485/files/texto_completo.pdf Versión publicada 2645444 http://zaguan.unizar.es/record/131485/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131485 articulos driver 2024-02-09-14:31:25 ARTICLE