000131495 001__ 131495 000131495 005__ 20240720100855.0 000131495 0247_ $$2doi$$a10.1016/j.biopha.2023.115703 000131495 0248_ $$2sideral$$a136901 000131495 037__ $$aART-2023-136901 000131495 041__ $$aeng 000131495 100__ $$aJurado, Paula 000131495 245__ $$aEssential oils of Pinus sylvestris, Citrus limon and Origanum vulgare exhibit high bactericidal and anti-biofilm activities against Neisseria gonorrhoeae and Streptococcus suis 000131495 260__ $$c2023 000131495 5060_ $$aAccess copy available to the general public$$fUnrestricted 000131495 5203_ $$aAntimicrobial resistance is a worldwide problem that urges novel alternatives to treat infections. In attempts to find novel molecules, we assess the antimicrobial potential of seven essential oils (EO) of different plants (Pinus sylvestris, Citrus limon, Origanum vulgare, Cymbopogon martini, Cinnamomum cassia, Melaleuca alternifolia and Eucalyptus globulus) against two multidrug-resistant bacteria species, i.e. Neisseria gonorrhoeae and Streptococcus suis. EOs of P. sylvestris and C. limon revealed higher bactericidal activity (MIC ≤ 0.5 mg/mL) and capacity to rapidly disperse biofilms of several N. gonorrhoeae clinical isolates than other EOs. Examination of biofilms exposed to both EO by electron microscopy revealed a reduction of bacterial aggregates, high production of extracellular vesicles, and alteration of cell integrity. This activity was dose-dependent and was enhanced in DNase I-treated biofilms. Antibiotic susceptibility studies confirmed that both EOs affected the outer membrane permeability, and analysis of EO- susceptibility of an LPS-deficient mutant suggested that both EO target the LPS bilayer. Further analysis revealed that α- and β-pinene and d-limonene, components of both EO, contribute to such activity. EO of C. martini, C. cassia, and O. vulgare exhibited promising antimicrobial activity (MIC ≤ 0.5 mg/mL) against S. suis, but only EO of O. vulgare exhibited a high biofilm dispersal activity, which was also confirmed by electron microscopy studies. To conclude, the EO of P. sylvestris, C. limon and O. vulgare studied in this work exhibit bactericidal and anti-biofilm activities against gonococcus and streptococcus, respectively. 000131495 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000131495 590__ $$a6.9$$b2023 000131495 592__ $$a1.493$$b2023 000131495 591__ $$aPHARMACOLOGY & PHARMACY$$b15 / 354 = 0.042$$c2023$$dQ1$$eT1 000131495 593__ $$aPharmacology$$c2023$$dQ1 000131495 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b21 / 189 = 0.111$$c2023$$dQ1$$eT1 000131495 593__ $$aMedicine (miscellaneous)$$c2023$$dQ1 000131495 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000131495 700__ $$0(orcid)0000-0002-2126-2232$$aUruén, Cristina$$uUniversidad de Zaragoza 000131495 700__ $$aMartínez, Sara 000131495 700__ $$aLain, Elena 000131495 700__ $$aSánchez, Sandra 000131495 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, Antonio 000131495 700__ $$aLópez, Víctor 000131495 700__ $$0(orcid)0000-0002-8134-0693$$aArenas, Jesús$$uUniversidad de Zaragoza 000131495 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000131495 773__ $$g168 (2023), 115703 [11 pp.]$$pBiomed. pharmacother.$$tBIOMEDICINE & PHARMACOTHERAPY$$x0753-3322 000131495 8564_ $$s4293601$$uhttps://zaguan.unizar.es/record/131495/files/texto_completo.pdf$$yVersión publicada 000131495 8564_ $$s2516504$$uhttps://zaguan.unizar.es/record/131495/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000131495 909CO $$ooai:zaguan.unizar.es:131495$$particulos$$pdriver 000131495 951__ $$a2024-07-19-18:50:40 000131495 980__ $$aARTICLE