131536 20240212142201.0 doi 10.1016/j.abb.2020.108323 sideral 117167 ART-2020-117167 eng García-Rubio, Inés Universidad de Zaragoza (orcid)0000-0002-1827-1250 EPR of site-directed spin-labeled proteins: A powerful tool to study structural flexibility 2020 Electron Paramagnetic Resonance is a spectroscopic technique which, in combination with site-directed spin-labeling, provides structural and dynamic information about proteins in conditions similar to those of their physiological environment. The information is sequence-resolved, as it is based on probing the local dynamics of a paramagnetic label incorporated as a side chain of a selected amino acid. EPR does not impose a limit on the size of the protein or protein complex, as long as it is amenable to site-directed mutagenesis, and is able to obtain reliable distance distributions between two or more labels (identical or different). The mean value, width and shape of distance distributions, as well as their dependence upon the state of the protein or interactions with physiological partners, provide insight into order-disorder transitions and the roles of protein flexibility. The main potentialities and limitations of the technique are revised and illustrated with examples of proteins for which order-disorder play an important role. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/E35-17R info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64486-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 4.013 2020 BIOPHYSICS 20 / 71 = 0.282 2020 Q2 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 136 / 296 = 0.459 2020 Q2 T2 1.097 2020 Biochemistry 2020 Q1 Molecular Biology 2020 Q1 Biophysics 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 684 (2020), 108323 [14 pp.] Arch. biochem. biophys. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 0003-9861 8245186 http://zaguan.unizar.es/record/131536/files/texto_completo.pdf Postprint 1620547 http://zaguan.unizar.es/record/131536/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:131536 articulos driver 2024-02-12-13:54:24 ARTICLE