000131560 001__ 131560
000131560 005__ 20240212142201.0
000131560 0247_ $$2doi$$a10.1016/j.diagmicrobio.2012.01.012
000131560 0248_ $$2sideral$$a76900
000131560 037__ $$aART-2012-76900
000131560 041__ $$aeng
000131560 100__ $$aDomingo, Maria P.
000131560 245__ $$aBis(methyl)Gliotoxin proves to be a more stable and reliable marker for invasive aspergillosis than gliotoxin and suitable for use in diagnosis
000131560 260__ $$c2012
000131560 5203_ $$aThe virulence factor gliotoxin (GT) and its inactive derivative, bis(methylthio)gliotoxin (bmGT), are produced by pathogens of the genus Aspergillus. Here we report the detection of GT and bmGT in serum of humans at risk of invasive aspergillosis (IA) as well as in cultures of fungal isolates derived from patients with proven infection with A. fumigatus. Although both compounds are readily recoverable from spiked human serum or plasma, only bmGT is retained in whole blood, indicating that bmGT may be the better marker for in vivo detection. Accordingly, bmGT was found more frequently than GT in samples from patients at risk of IA and incultures of clinical isolates of A. fumigatus. In some cases, bmGT was detected before mycologic evidence ofinfection was gained. Importantly, neither GT nor bmGT was found in serum from healthy donors or from neutropenic patients without any sign of infection. Thus, bmGT presence might provide a more reliable indicator of A. fumigatus infections than GT. Due to its simplicity and sensitivity, a diagnostic technology based on this test could be easily adopted in clinical laboratories to help in the diagnosis of this often fatal fungal infection.
000131560 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000131560 590__ $$a2.26$$b2012
000131560 591__ $$aMICROBIOLOGY$$b62 / 116 = 0.534$$c2012$$dQ3$$eT2
000131560 591__ $$aINFECTIOUS DISEASES$$b45 / 70 = 0.643$$c2012$$dQ3$$eT2
000131560 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000131560 700__ $$aColmenarejo, Cristina
000131560 700__ $$0(orcid)0000-0003-3043-147X$$aMartínez-Lostao, Luis$$uUniversidad de Zaragoza
000131560 700__ $$aMüllbacherd, Arno
000131560 700__ $$0(orcid)0000-0002-8701-9745$$aJarne Lardiés, Carmen
000131560 700__ $$aRevillo, María J.
000131560 700__ $$aDelgado, Pilar
000131560 700__ $$aRoc, Lourdes
000131560 700__ $$aMeis, Jacques F.
000131560 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, Antonio$$uUniversidad de Zaragoza
000131560 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, Julian$$uUniversidad de Zaragoza
000131560 700__ $$0(orcid)0000-0001-6928-5516$$aGálvez, Eva M.
000131560 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000131560 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000131560 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000131560 773__ $$g73 (2012), 57-64$$pDiagn. microbiol. infect. dis.$$tDiagnostic Microbiology and Infectious Disease$$x0732-8893
000131560 8564_ $$s1116317$$uhttps://zaguan.unizar.es/record/131560/files/texto_completo.pdf$$yVersión publicada
000131560 8564_ $$s1144930$$uhttps://zaguan.unizar.es/record/131560/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000131560 909CO $$ooai:zaguan.unizar.es:131560$$particulos$$pdriver
000131560 951__ $$a2024-02-12-13:56:19
000131560 980__ $$aARTICLE