131560 20240212142201.0 doi 10.1016/j.diagmicrobio.2012.01.012 sideral 76900 ART-2012-76900 eng Domingo, Maria P. Bis(methyl)Gliotoxin proves to be a more stable and reliable marker for invasive aspergillosis than gliotoxin and suitable for use in diagnosis 2012 The virulence factor gliotoxin (GT) and its inactive derivative, bis(methylthio)gliotoxin (bmGT), are produced by pathogens of the genus Aspergillus. Here we report the detection of GT and bmGT in serum of humans at risk of invasive aspergillosis (IA) as well as in cultures of fungal isolates derived from patients with proven infection with A. fumigatus. Although both compounds are readily recoverable from spiked human serum or plasma, only bmGT is retained in whole blood, indicating that bmGT may be the better marker for in vivo detection. Accordingly, bmGT was found more frequently than GT in samples from patients at risk of IA and incultures of clinical isolates of A. fumigatus. In some cases, bmGT was detected before mycologic evidence ofinfection was gained. Importantly, neither GT nor bmGT was found in serum from healthy donors or from neutropenic patients without any sign of infection. Thus, bmGT presence might provide a more reliable indicator of A. fumigatus infections than GT. Due to its simplicity and sensitivity, a diagnostic technology based on this test could be easily adopted in clinical laboratories to help in the diagnosis of this often fatal fungal infection. info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.26 2012 MICROBIOLOGY 62 / 116 = 0.534 2012 Q3 T2 INFECTIOUS DISEASES 45 / 70 = 0.643 2012 Q3 T2 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Colmenarejo, Cristina Martínez-Lostao, Luis Universidad de Zaragoza (orcid)0000-0003-3043-147X Müllbacherd, Arno Jarne Lardiés, Carmen (orcid)0000-0002-8701-9745 Revillo, María J. Delgado, Pilar Roc, Lourdes Meis, Jacques F. Rezusta, Antonio Universidad de Zaragoza (orcid)0000-0001-7294-245X Pardo, Julian Universidad de Zaragoza (orcid)0000-0003-0154-0730 Gálvez, Eva M. (orcid)0000-0001-6928-5516 1008 630 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Microbiología 1008 566 Universidad de Zaragoza Dpto. Microb.Med.Pr.,Sal.Públ. Área Inmunología 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 73 (2012), 57-64 Diagn. microbiol. infect. dis. Diagnostic Microbiology and Infectious Disease 0732-8893 1116317 http://zaguan.unizar.es/record/131560/files/texto_completo.pdf Versión publicada 1144930 http://zaguan.unizar.es/record/131560/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131560 articulos driver 2024-02-12-13:56:19 ARTICLE