131615 20241125101139.0 doi 10.1021/acsnano.3c06172 sideral 136985 ART-2023-136985 eng Cappetta, Elisa An integrated multilevel approach unveils complex seed–nanoparticle interactions and their implications for seed priming 2023 Nanotechnology has the potential to revolutionize agriculture with the introduction of engineered nanomaterials. However, their use is hindered by high cost, marginal knowledge of their interactions with plants, and unpredictable effects related to massive use in crop cultivation. Nanopriming is an innovative seed priming technology able to match economic, agronomic, and environmental needs in agriculture. The present study was focused on unveiling, by a multilevel integrated approach, undisclosed aspects of seed priming mediated by iron oxide magnetic nanoparticles in pepper seeds (Capsicum annuum), one of the most economically important crops worldwide. Inductively coupled plasma atomic emission mass spectrometry and scanning electron microscopy were used to quantify the MNP uptake and assess seed surface changes. Magnetic resonance imaging mapped the distribution of MNPs prevalently in the seed coat. The application of MNPs significantly enhanced the root and vegetative growth of pepper plants, whereas seed priming with equivalent Fe concentrations supplied as FeCl3 did not yield these positive effects. Finally, global gene expression by RNA-sequencing identified more than 2,200 differentially expressed genes, most of them involved in plant developmental processes and defense mechanisms. Collectively, these data provide evidence on the link between structural seed changes and an extensive transcriptional reprogramming, which boosts the plant growth and primes the embryo to cope with environmental challenges that might occur during the subsequent developmental and growth stages. info:eu-repo/grantAgreement/EC/H2020/853468/EU/Remote control of cellular signalling triggered by magnetic switching/SIROCCO This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 853468-SIROCCO info:eu-repo/grantAgreement/ES/MICINN/RYC2019-026860-I info:eu-repo/semantics/closedAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 15.8 2023 CHEMISTRY, PHYSICAL 14 / 178 = 0.079 2023 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 11 / 141 = 0.078 2023 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 15 / 231 = 0.065 2023 Q1 T1 MATERIALS SCIENCE, MULTIDISCIPLINARY 27 / 439 = 0.062 2023 Q1 T1 4.593 2023 Engineering (miscellaneous) 2023 Q1 Physics and Astronomy (miscellaneous) 2023 Q1 Nanoscience and Nanotechnology 2023 Q1 Materials Science (miscellaneous) 2023 Q1 26.0 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Del Regno, Carmine Conte, Marisa Castro-Hinojosa, Christian Del Sol-Fernández, Susel Vergata, Chiara Buti, Matteo Curcio, Rossella Onder, Anil Mazzei, Pierluigi Funicello, Nicola De Pasquale, Salvatore Terzaghi, Mattia Del Gaudio, Pasquale Leone, Antonietta Martinelli, Federico Moros, Maria (orcid)0000-0002-2861-2469 Ambrosone, Alfredo 17, 22 (2023), 22539-22552 ACS Nano ACS NANO 1936-0851 11369849 http://zaguan.unizar.es/record/131615/files/texto_completo.pdf Versión publicada 3146843 http://zaguan.unizar.es/record/131615/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131615 articulos driver 2024-11-22-12:01:51 ARTICLE