000131661 001__ 131661 000131661 005__ 20240219150720.0 000131661 0247_ $$2doi$$a10.1529/biophysj.108.133272 000131661 0248_ $$2sideral$$a128992 000131661 037__ $$aART-2009-128992 000131661 041__ $$aeng 000131661 100__ $$0(orcid)0000-0002-1827-1250$$aGarcía-Rubio, Inés 000131661 245__ $$aHyperfine Correlation Spectroscopy and Electron Spin Echo Envelope Modulation Spectroscopy Study of the Two Coexisting Forms of the Hemeprotein Cytochrome c6 from Anabaena Pcc7119 000131661 260__ $$c2009 000131661 5060_ $$aAccess copy available to the general public$$fUnrestricted 000131661 5203_ $$aOxidized cytochrome from Anabaena PCC 7119 was studied by electron spin echo envelope modulation spectroscopy. Hyperfine couplings of the unpaired electron with several nuclei were detected, in particular those of the nitrogens bound to the iron atom. Combining the experimental information here presented and previous continuous wave-electron paramagnetic resonance and electron nuclear double resonance results, some details on the electronic structure of the heme center in the protein are obtained. These results are discussed on the basis of a molecular model that considers one unpaired electron localized mainly in the iron d orbitals and propagation of the spin density within the heme center via spin polarization of the nitrogen σ-orbitals. The coexistence of two heme forms at physiological pH values in this c-type cytochrome is also discussed taking into account the experimental evidence. 000131661 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000131661 590__ $$a4.39$$b2009 000131661 591__ $$aBIOPHYSICS$$b10 / 74 = 0.135$$c2009$$dQ1$$eT1 000131661 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000131661 700__ $$aAlonso, Pablo J. 000131661 700__ $$0(orcid)0000-0001-8743-0182$$aMedina, Milagros$$uUniversidad de Zaragoza 000131661 700__ $$0(orcid)0000-0002-5406-3280$$aMartínez, Jesús I.$$uUniversidad de Zaragoza 000131661 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000131661 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada 000131661 773__ $$g96, 1 (2009), 141-152$$pBiophys. j.$$tBIOPHYSICAL JOURNAL$$x0006-3495 000131661 8564_ $$s419917$$uhttps://zaguan.unizar.es/record/131661/files/texto_completo.pdf$$yVersión publicada 000131661 8564_ $$s3195362$$uhttps://zaguan.unizar.es/record/131661/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000131661 909CO $$ooai:zaguan.unizar.es:131661$$particulos$$pdriver 000131661 951__ $$a2024-02-19-13:22:35 000131661 980__ $$aARTICLE