131661 20240219150720.0 doi 10.1529/biophysj.108.133272 sideral 128992 ART-2009-128992 eng García-Rubio, Inés (orcid)0000-0002-1827-1250 Hyperfine Correlation Spectroscopy and Electron Spin Echo Envelope Modulation Spectroscopy Study of the Two Coexisting Forms of the Hemeprotein Cytochrome c6 from Anabaena Pcc7119 2009 Oxidized cytochrome from Anabaena PCC 7119 was studied by electron spin echo envelope modulation spectroscopy. Hyperfine couplings of the unpaired electron with several nuclei were detected, in particular those of the nitrogens bound to the iron atom. Combining the experimental information here presented and previous continuous wave-electron paramagnetic resonance and electron nuclear double resonance results, some details on the electronic structure of the heme center in the protein are obtained. These results are discussed on the basis of a molecular model that considers one unpaired electron localized mainly in the iron d orbitals and propagation of the spin density within the heme center via spin polarization of the nitrogen σ-orbitals. The coexistence of two heme forms at physiological pH values in this c-type cytochrome is also discussed taking into account the experimental evidence. Access copy available to the general public Unrestricted info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.39 2009 BIOPHYSICS 10 / 74 = 0.135 2009 Q1 T1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Alonso, Pablo J. Medina, Milagros Universidad de Zaragoza (orcid)0000-0001-8743-0182 Martínez, Jesús I. Universidad de Zaragoza (orcid)0000-0002-5406-3280 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 96, 1 (2009), 141-152 Biophys. j. BIOPHYSICAL JOURNAL 0006-3495 419917 http://zaguan.unizar.es/record/131661/files/texto_completo.pdf Versión publicada 3195362 http://zaguan.unizar.es/record/131661/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131661 articulos driver 2024-02-19-13:22:35 ARTICLE