131670 20241125101141.0 doi 10.1016/j.cej.2023.146679 sideral 137197 ART-2023-137197 eng Miranda-Calderon, Laura Universidad de Zaragoza Combinatorial wound dressings loaded with synergistic antibiotics in the treatment of chronic infected wounds 2023 Access copy available to the general public Unrestricted Advanced medicated wound dressings fabricated by electrospinning and electrospraying were prepared for the eradication of topical bacterial infections potentially applied in the management of infected acute and chronic non-healing wounds. Two different antibiotics (ciprofloxacin and rifampicin), with different aqueous solubilities and different mechanisms of antimicrobial action, were loaded within electrosprayed polymer microparticles and within electrospun nanofibers, respectively, to provide the resulting wound dressing with dually controlled antibiotic release kinetics. Due to the large surface area per volume ratio of the electrosprayed microparticles containing ciprofloxacin, an initial burst release was obtained. Simultaneously, the reduced surface area per volume ratio for the electrospun nanofibers together with the reduced aqueous solubility of rifampicin produced an extended rifampicin release over time. More importantly, a synergistic antimicrobial effect against Gram-positive and Gram-negative bacteria was observed when both antibiotics were combined. Biofilm formation prevention and the elimination of already formed mature bacterial biofilms were also successfully achieved using our advanced dressings. The lack of cytotoxicity of the advanced wound dressings here reported against eukaryotic cells at antimicrobial doses was also demonstrated using three different mammalian cell lines. Moreover, the advanced wound dressings successfully eliminated a Staphylococcus aureus mediated experimental infection in a chronic wound murine model showing their efficacy for the treatment of these complicated non-healing wounds. The strategy of advanced medicated wound dressings developed here may be used as a potential methodology for the fabrication of functional combinatorial materials that offer the ability to eradicate bacterial infections. info:eu-repo/grantAgreement/ES/AEI/PDC2021-121105-I00 info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092 info:eu-repo/grantAgreement/ES/MCINN/PRE2018-085769 info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 13.4 2023 ENGINEERING, ENVIRONMENTAL 3 / 81 = 0.037 2023 Q1 T1 ENGINEERING, CHEMICAL 7 / 170 = 0.041 2023 Q1 T1 2.852 2023 Environmental Chemistry 2023 Q1 Industrial and Manufacturing Engineering 2023 Q1 Chemistry (miscellaneous) 2023 Q1 Chemical Engineering (miscellaneous) 2023 Q1 21.7 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Yus, Cristina Universidad de Zaragoza (orcid)0000-0002-1505-498X Remirez de Ganuza, Cristina Universidad de Zaragoza Paesa, Monica Universidad de Zaragoza Landa, Guillermo Universidad de Zaragoza (orcid)0000-0003-1599-8216 Tapia, Elena Universidad de Zaragoza Pérez, Estela Universidad de Zaragoza Perez, Marta Universidad de Zaragoza (orcid)0000-0002-8133-2124 Sebastian, Victor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Irusta, Silvia Universidad de Zaragoza (orcid)0000-0002-2966-9088 Mendoza, Gracia (orcid)0000-0003-2293-363X Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 0 X Universidad de Zaragoza Serv.Gral. Apoyo Investigación Servicios. División Biomédica 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 476 (2023), 146679 [15 pp.] Chem. eng. j. Chemical Engineering Journal 1385-8947 13287800 http://zaguan.unizar.es/record/131670/files/texto_completo.pdf Versión publicada 2383897 http://zaguan.unizar.es/record/131670/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131670 articulos driver 2024-11-22-12:02:36 ARTICLE