131691 20250908131417.0 doi 10.1016/j.ijpharm.2023.123502 sideral 137122 ART-2023-137122 eng Landa, Guillermo Universidad de Zaragoza (orcid)0000-0003-1599-8216 Real-time in vivo monitoring of the antimicrobial action of combination therapies in the management of infected topical wounds 2023 Access copy available to the general public Unrestricted The increasing prevalence of non-healing infected wounds has become a serious concern in the clinical practice, being associated to population aging and to the rising prevalence of several chronic conditions such as diabetes. Herein, the evaluation of the bactericidal and antibiofilm effects of the natural antiseptic terpenes thymol and farnesol standing alone or in combination with the standard care antiseptic chlorhexidine was carried out both in vitro and in vivo. The in vitro combinatorial treatment of chlorhexidine associated with those terpenes against Staphylococcus aureus in its planktonic and sessile forms demonstrated a superior antibacterial activity than that of chlorhexidine alone. Real-time in vivo monitoring of infection progression and antimicrobial treatment outcomes were evaluated using the bioluminescent S. aureus strain Xen36. In vivo studies on infected wound splinting murine models corroborated the superior bactericidal effects of the combinatorial treatments here proposed. Moreover, the encapsulation of thymol in electrospun Eudragit® S100 (i.e., a synthetic anionic copolymer of methacrylic acid and ethyl acrylate)-based wound dressings was also carried out in order to design efficient antimicrobial wound dressings. info:eu-repo/grantAgreement/ES/ISCIII/MS19-00092 info:eu-repo/grantAgreement/ES/MCINN/PRE2018-085769 info:eu-repo/grantAgreement/ES/MICINN/PDC2021-121405-I00 info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 5.3 2023 PHARMACOLOGY & PHARMACY 35 / 354 = 0.099 2023 Q1 T1 0.954 2023 Pharmaceutical Science 2023 Q1 10.7 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Miranda-Calderón, Laura G. Universidad de Zaragoza Gómez, Alex Universidad de Zaragoza (orcid)0000-0002-9723-9004 Pérez, Marta Universidad de Zaragoza (orcid)0000-0002-8133-2124 Sebastian, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Lamarche, Isabelle Tewes, Frederic Irusta, Silvia Universidad de Zaragoza (orcid)0000-0002-2966-9088 Mendoza, Gracia (orcid)0000-0003-2293-363X 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 5005 790 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Tecnologi. Medio Ambiente 646 (2023), 123502 [10 pp.] Int. j. pharm. International Journal of Pharmaceutics 0378-5173 10184635 http://zaguan.unizar.es/record/131691/files/texto_completo.pdf Versión publicada 2528396 http://zaguan.unizar.es/record/131691/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131691 articulos driver 2025-09-08-12:52:07 ARTICLE