000131843 001__ 131843
000131843 005__ 20240219150722.0
000131843 0247_ $$2doi$$a10.3390/ani14030481
000131843 0248_ $$2sideral$$a137256
000131843 037__ $$aART-2024-137256
000131843 041__ $$aeng
000131843 100__ $$0(orcid)0000-0002-2497-9028$$aBenito, Alfredo A.
000131843 245__ $$aMolecular diagnosis of footrot and contagious ovine digital dermatitis in small ruminants in the Iberian Peninsula
000131843 260__ $$c2024
000131843 5060_ $$aAccess copy available to the general public$$fUnrestricted
000131843 5203_ $$aContagious ovine digital dermatitis (CODD) and footrot (FR), a sub-acute or acute necrotic (decaying) infectious disease involving the hoof and underlying tissues, pose economic challenges to herds in Spain and worldwide. The aetiological agent for FR is Dichelobacter nodosus, while CODD is caused by pathogenic Treponema phylogroups. We detail the findings derived from the analysis by qPCR of 105 pooled samples from 100 ovine and five caprine herds in Spain and Portugal, alongside 15 samples from healthy flocks in order to identify Dichelobacter nodosus, Fusobacterium necrophorum, Treponema spp., and three pathogenic Treponema phylogroups (T. phagedenis, T. medium, and T. pedis). Treponema spp. were detected in all 120 pools, including samples from the 15 healthy flocks where only one positive result for F. necrophorum was recorded. Mixed infections by agents different from Treponema spp. were identified in 68.57% of samples. Positive results for F. necrophorum and/or D. nodosus, were obtained for 91.4% of the pools, whereas the presence of the three pathogenic Treponema phylogroups was rare: each of them appeared in isolation in a single pool, while they were found in 18 pools in combination with other agents. While F. necrophorum was the sole finding in 16.2% of samples from affected herds, D. nodosus (the footrot causative agent) was only detected in 61% of affected farms. An improved qPCR protocol was implemented to determine the serogroups of D. nodosus in the samples and found all of them (except the G serogroup), often in combined infections (35.1%). This report concludes with comprehensive proposals for diagnosing, preventing, and treating hoof ailments, remarking the interest of the information about D. nodosus serogroups in order to improve the efficiency of immunization by choosing appropriate vaccine protocols.
000131843 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000131843 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000131843 700__ $$aAnía, Silvia
000131843 700__ $$0(orcid)0000-0002-6282-5038$$aRamo, María de los Ángeles$$uUniversidad de Zaragoza
000131843 700__ $$aBaselga, Cristina
000131843 700__ $$0(orcid)0000-0001-7831-2483$$aQuílez, Joaquín$$uUniversidad de Zaragoza
000131843 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, María Teresa$$uUniversidad de Zaragoza
000131843 700__ $$0(orcid)0000-0003-0210-2919$$aMonteagudo, Luis Vicente$$uUniversidad de Zaragoza
000131843 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000131843 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000131843 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000131843 773__ $$g14, 481 (2024), 1-10$$pAnimals (Basel)$$tAnimals$$x2076-2615
000131843 8564_ $$s252030$$uhttps://zaguan.unizar.es/record/131843/files/texto_completo.pdf$$yVersión publicada
000131843 8564_ $$s3037490$$uhttps://zaguan.unizar.es/record/131843/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000131843 909CO $$ooai:zaguan.unizar.es:131843$$particulos$$pdriver
000131843 951__ $$a2024-02-19-13:28:40
000131843 980__ $$aARTICLE