131843 20260217205512.0 doi 10.3390/ani14030481 sideral 137256 ART-2024-137256 eng Benito, Alfredo A. (orcid)0000-0002-2497-9028 Molecular diagnosis of footrot and contagious ovine digital dermatitis in small ruminants in the Iberian Peninsula 2024 Access copy available to the general public Unrestricted Contagious ovine digital dermatitis (CODD) and footrot (FR), a sub-acute or acute necrotic (decaying) infectious disease involving the hoof and underlying tissues, pose economic challenges to herds in Spain and worldwide. The aetiological agent for FR is Dichelobacter nodosus, while CODD is caused by pathogenic Treponema phylogroups. We detail the findings derived from the analysis by qPCR of 105 pooled samples from 100 ovine and five caprine herds in Spain and Portugal, alongside 15 samples from healthy flocks in order to identify Dichelobacter nodosus, Fusobacterium necrophorum, Treponema spp., and three pathogenic Treponema phylogroups (T. phagedenis, T. medium, and T. pedis). Treponema spp. were detected in all 120 pools, including samples from the 15 healthy flocks where only one positive result for F. necrophorum was recorded. Mixed infections by agents different from Treponema spp. were identified in 68.57% of samples. Positive results for F. necrophorum and/or D. nodosus, were obtained for 91.4% of the pools, whereas the presence of the three pathogenic Treponema phylogroups was rare: each of them appeared in isolation in a single pool, while they were found in 18 pools in combination with other agents. While F. necrophorum was the sole finding in 16.2% of samples from affected herds, D. nodosus (the footrot causative agent) was only detected in 61% of affected farms. An improved qPCR protocol was implemented to determine the serogroups of D. nodosus in the samples and found all of them (except the G serogroup), often in combined infections (35.1%). This report concludes with comprehensive proposals for diagnosing, preventing, and treating hoof ailments, remarking the interest of the information about D. nodosus serogroups in order to improve the efficiency of immunization by choosing appropriate vaccine protocols. info:eu-repo/semantics/openAccess by https://creativecommons.org/licenses/by/4.0/deed.es 2.7 2024 VETERINARY SCIENCES 21 / 170 = 0.124 2024 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 15 / 86 = 0.174 2024 Q1 T1 0.733 2024 Veterinary (miscellaneous) 2024 Q1 Animal Science and Zoology 2024 Q1 5.2 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Anía, Silvia Ramo, María de los Ángeles Universidad de Zaragoza (orcid)0000-0002-6282-5038 Baselga, Cristina Quílez, Joaquín Universidad de Zaragoza (orcid)0000-0001-7831-2483 Tejedor, María Teresa Universidad de Zaragoza (orcid)0000-0001-5026-5144 Monteagudo, Luis Vicente Universidad de Zaragoza (orcid)0000-0003-0210-2919 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 14, 481 (2024), 1-10 Animals (Basel) Animals 2076-2615 252030 http://zaguan.unizar.es/record/131843/files/texto_completo.pdf Versión publicada 3037490 http://zaguan.unizar.es/record/131843/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:131843 articulos driver 2026-02-17-20:25:40 ARTICLE