DNA transfection to mesenchymal stem cells using a novel type of pseudodendrimer based on 2,2-bis(hydroxymethyl)propionic acid

Lancelot, A. (Universidad de Zaragoza) ; González-Pastor, R. (Universidad de Zaragoza) ; Concellón, A. (Universidad de Zaragoza) ; Sierra, T. (Universidad de Zaragoza) ; Martín-Duque, P. ; Serrano, J. L. (Universidad de Zaragoza)

Resumen: In the search for effective vehicles to carry genetic material into cells, we present here new pseudodendrimers that consist of a hyperbranched polyester core surrounded by amino-terminated 2,2-bis(hydroxymethyl)propionic acid (bis-MPA) dendrons. The pseudodendrimers are readily synthesized from commercial hyperbranched bis-MPA polyesters of the second, third, and fourth generations and third-generation bis-MPA dendrons, bearing eight peripheral glycine moieties, coupled by the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). This approach provides globular macromolecular structures bearing 128, 256, and 512 terminal amino groups, and these can complex pDNA. The toxicity of the three pseudodendrimers was studied on two cell lines, mesenchymal stem cells, and HeLa, and it was demonstrated that these compounds do not affect negatively cell viability up to 72 h. The complexation with DNA was investigated in terms of N-to-P ratio and dendriplex stability. The three generations were found to promote internalizing of pDNA into mesenchymal stem cells (MSCs), and their transfection capacity was compared with two nonviral commercial transfection agents, Lipofectamine and TransIT-X2. The highest generations were able to transfect these cells at levels comparable to both commercial reagents.

Idioma: Inglés

DOI: 10.1021/acs.bioconjchem.7b00037

Año: 2017

Publicado en: Bioconjugate Chemistry 28, 4 (2017), 1135-1150
ISSN: 1043-1802

Factor impacto JCR: 4.485 (2017)

Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 10 / 77 = 0.13 (2017) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 42 / 169 = 0.249 (2017) - Q1 - T1
Categ. JCR: CHEMISTRY, ORGANIC rank: 10 / 57 = 0.175 (2017) - Q1 - T1
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 61 / 292 = 0.209 (2017) - Q1 - T1

Factor impacto SCIMAGO: 1.801 - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmacology (Q1) - Organic Chemistry (Q1) - Pharmaceutical Science (Q1) - Biotechnology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E04
Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU12-05210
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70174-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2015-66208-C3-1-P

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)