132065 20240301161205.0 doi 10.1021/acs.bioconjchem.7b00037 sideral 98873 ART-2017-98873 eng Lancelot, A. Universidad de Zaragoza (orcid)0000-0001-6584-6603 DNA transfection to mesenchymal stem cells using a novel type of pseudodendrimer based on 2,2-bis(hydroxymethyl)propionic acid 2017 In the search for effective vehicles to carry genetic material into cells, we present here new pseudodendrimers that consist of a hyperbranched polyester core surrounded by amino-terminated 2,2-bis(hydroxymethyl)propionic acid (bis-MPA) dendrons. The pseudodendrimers are readily synthesized from commercial hyperbranched bis-MPA polyesters of the second, third, and fourth generations and third-generation bis-MPA dendrons, bearing eight peripheral glycine moieties, coupled by the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). This approach provides globular macromolecular structures bearing 128, 256, and 512 terminal amino groups, and these can complex pDNA. The toxicity of the three pseudodendrimers was studied on two cell lines, mesenchymal stem cells, and HeLa, and it was demonstrated that these compounds do not affect negatively cell viability up to 72 h. The complexation with DNA was investigated in terms of N-to-P ratio and dendriplex stability. The three generations were found to promote internalizing of pDNA into mesenchymal stem cells (MSCs), and their transfection capacity was compared with two nonviral commercial transfection agents, Lipofectamine and TransIT-X2. The highest generations were able to transfect these cells at levels comparable to both commercial reagents. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/E04 info:eu-repo/grantAgreement/ES/MEC/FPU12-05210 info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70174-P info:eu-repo/grantAgreement/ES/MINECO/MAT2015-66208-C3-1-P info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.485 2017 BIOCHEMICAL RESEARCH METHODS 10 / 77 = 0.13 2017 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 42 / 169 = 0.249 2017 Q1 T1 CHEMISTRY, ORGANIC 10 / 57 = 0.175 2017 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 61 / 292 = 0.209 2017 Q1 T1 1.801 2017 Bioengineering 2017 Q1 Biomedical Engineering 2017 Q1 Pharmacology 2017 Q1 Organic Chemistry 2017 Q1 Pharmaceutical Science 2017 Q1 Biotechnology 2017 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion González-Pastor, R. Universidad de Zaragoza (orcid)0000-0002-5630-6645 Concellón, A. Universidad de Zaragoza (orcid)0000-0002-8932-9085 Sierra, T. Universidad de Zaragoza (orcid)0000-0001-7091-077X Martín-Duque, P. (orcid)0000-0003-4160-9720 Serrano, J. L. Universidad de Zaragoza (orcid)0000-0001-9866-6633 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 28, 4 (2017), 1135-1150 Bioconjug. chem. Bioconjugate Chemistry 1043-1802 2403325 http://zaguan.unizar.es/record/132065/files/texto_completo.pdf Postprint 922608 http://zaguan.unizar.es/record/132065/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:132065 articulos driver 2024-03-01-14:36:38 ARTICLE