000132120 001__ 132120 000132120 005__ 20240301161206.0 000132120 0247_ $$2doi$$a10.1021/acscentsci.1c00173 000132120 0248_ $$2sideral$$a131575 000132120 037__ $$aART-2021-131575 000132120 041__ $$aeng 000132120 100__ $$aLi, Jie 000132120 245__ $$aJanus emulsion biosensors for anti-SARS-CoV-2 spike antibody 000132120 260__ $$c2021 000132120 5060_ $$aAccess copy available to the general public$$fUnrestricted 000132120 5203_ $$aThe spread of the COVID-19 pandemic around the world has revealed that it is urgently important to develop rapid and inexpensive assays for antibodies in general and anti-SARS-CoV-2 IgG antibody (anti-SARS-CoV-2 spike glycoprotein S1 antibody) in particular. Herein we report a method to detect the anti-SARS-CoV-2 spike antibody level by using Janus emulsions or Janus particles as biosensors. Janus emulsions are composed of two immiscible hydrocarbon and fluorocarbon oils. The hydrocarbon/water interfaces are functionalized with a secondary antibody of IgG protein and SARS-CoV-2 spike receptor binding domain (RBD), to produce two different Janus emulsions. Mixtures of these Janus droplets enable the detection of the anti-SARS-CoV-2 spike IgG antibody in an agglutination assay caused by the antibody’s binding to both the secondary antibody of IgG antibody and SARS-CoV-2 spike protein RBD. Both qualitative optical images and quantitative fluorescence spectra are able to detect the level of anti-SARS-CoV-2 spike antibody at concentrations as low as 0.2 µg/mL in 2 h. The detection results of clinical human serum samples using this agglutination assay confirm that this method is applicable to clinical samples with good sensitivity and specificity. The reported method is generalizable and can be used to detect other analytes by attaching different biomolecular recognition elements to the surface of the Janus droplets. 000132120 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000132120 590__ $$a18.728$$b2021 000132120 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b12 / 179 = 0.067$$c2021$$dQ1$$eT1 000132120 592__ $$a4.093$$b2021 000132120 593__ $$aChemistry (miscellaneous)$$c2021$$dQ1 000132120 593__ $$aChemical Engineering (miscellaneous)$$c2021$$dQ1 000132120 594__ $$a21.4$$b2021 000132120 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000132120 700__ $$0(orcid)0000-0002-8932-9085$$aConcellón, Alberto 000132120 700__ $$aYoshinaga, Kosuke 000132120 700__ $$aNelson, Zachary 000132120 700__ $$aHe, Qilin 000132120 700__ $$aSwager, Timothy M. 000132120 773__ $$g7, 7 (2021), 1166-1175$$pACS cent. sci.$$tACS Central Science$$x2374-7943 000132120 8564_ $$s11055540$$uhttps://zaguan.unizar.es/record/132120/files/texto_completo.pdf$$yVersión publicada 000132120 8564_ $$s3186910$$uhttps://zaguan.unizar.es/record/132120/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000132120 909CO $$ooai:zaguan.unizar.es:132120$$particulos$$pdriver 000132120 951__ $$a2024-03-01-14:49:49 000132120 980__ $$aARTICLE