000132131 001__ 132131
000132131 005__ 20250923084416.0
000132131 0247_ $$2doi$$a10.1016/j.ijbiomac.2024.129541
000132131 0248_ $$2sideral$$a137085
000132131 037__ $$aART-2024-137085
000132131 041__ $$aeng
000132131 100__ $$aMatarredona, Laura
000132131 245__ $$aGlobal Lrp regulator protein from Haloferax mediterranei: Transcriptional analysis and structural characterization
000132131 260__ $$c2024
000132131 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132131 5203_ $$aHaloferax mediterranei, an extreme halophilic archaeon thriving in hypersaline environments, has acquired significant attention in biotechnological and biochemical research due to its remarkable ability to flourish in extreme salinity conditions. Transcription factors, essential in regulating diverse cellular processes, have become focal points in understanding its adaptability. This study delves into the role of the Lrp transcription factor, exploring its modulation of glnA, nasABC, and lrp gene promoters in vivo through β-galactosidase assays. Remarkably, our findings propose Lrp as the pioneering transcriptional regulator of nitrogen metabolism identified in a haloarchaeon. This study suggests its potential role in activating or repressing assimilatory pathway enzymes (GlnA and NasA). The interaction between Lrp and these promoters is analyzed using Electrophoretic Mobility Shift Assay and Differential Scanning Fluorimetry, highlighting l-glutamine's indispensable role in stabilizing the Lrp-DNA complex. Our research uncovers that halophilic Lrp forms octameric structures in the presence of l-glutamine. The study reveals the three-dimensional structure of the Lrp as a homodimer using X-ray crystallography, confirming this state in solution by Small-Angle X-ray Scattering. These findings illuminate the complex molecular mechanisms driving Hfx. mediterranei's nitrogen metabolism, offering valuable insights about its gene expression regulation and enriching our comprehension of extremophile biology.
000132131 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/BIO2013-42921-P
000132131 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000132131 590__ $$a8.5$$b2024
000132131 592__ $$a1.285$$b2024
000132131 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b29 / 319 = 0.091$$c2024$$dQ1$$eT1
000132131 593__ $$aBiochemistry$$c2024$$dQ1
000132131 591__ $$aPOLYMER SCIENCE$$b6 / 94 = 0.064$$c2024$$dQ1$$eT1
000132131 593__ $$aBiomaterials$$c2024$$dQ1
000132131 591__ $$aCHEMISTRY, APPLIED$$b8 / 75 = 0.107$$c2024$$dQ1$$eT1
000132131 593__ $$aFood Science$$c2024$$dQ1
000132131 593__ $$aMolecular Biology$$c2024$$dQ2
000132131 593__ $$aStructural Biology$$c2024$$dQ2
000132131 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000132131 700__ $$aGarcía-Bonete, María José
000132131 700__ $$0(orcid)0000-0002-0474-255X$$aGuío, Jorge
000132131 700__ $$aCamacho, Mónica
000132131 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, María F.$$uUniversidad de Zaragoza
000132131 700__ $$aEsclapez, Julia
000132131 700__ $$aBonete, María José
000132131 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000132131 773__ $$g260, Parte 2 (2024), 129541 [12 pp.]$$pInt. j. biol. macromol.$$tInternational journal of biological macromolecules$$x0141-8130
000132131 8564_ $$s9188423$$uhttps://zaguan.unizar.es/record/132131/files/texto_completo.pdf$$yVersión publicada
000132131 8564_ $$s2634545$$uhttps://zaguan.unizar.es/record/132131/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000132131 909CO $$ooai:zaguan.unizar.es:132131$$particulos$$pdriver
000132131 951__ $$a2025-09-22-14:33:20
000132131 980__ $$aARTICLE