132133 20241125101140.0 doi 10.1016/j.talanta.2023.125119 sideral 137298 ART-2023-137298 eng Navarro, Jesús Towards new fluorometric methodologies based on the in-situ generation of gold nanoclusters 2023 Access copy available to the general public Unrestricted In this manuscript a method for the fluorometric determination of tyramine is described. It is based on the direct reaction between Au(III) and tyramine in a phosphate buffer which produces fluorescent gold nanoclusters (AuNC) (λexc = 320 nm, λem = 410 nm) with a diameter of 1.50 ± 0.06 nm. The Au(III) and buffer solutions are mixed and after 140 s, tyramine solution is added; which produces a fast and stable fluorescence signal. The formation of AuNC is demonstrated by STEM and, more importantly, this reaction could be followed by Atomic Fluorescence Microscopy (AFM). The method allows the determination of tyramine in the range from 6.0x10−7 M (limit of quantification) up to 1.2x10−4 M; with a relative standard deviation (RSD) ranges from 1.8% to 4.4% depending on the tyramine concentration. The mechanism of AuNC formation involves the Au(III) reduction via the phenol group and the complexation with the amine group. Putrescine and cadaverine do not produce interference, meanwhile histamine causes a proportional decrease in the signal which can be overcome by the standard addition method. The method was applied to the determination of tyramine in a tuna and cheese samples and the results obtained are in statistical agreement with these obtained using a validated or standard method. info:eu-repo/grantAgreement/ES/DGA/E25-20R info:eu-repo/grantAgreement/ES/DGA/E31-20R info:eu-repo/grantAgreement/ES/DGA/LMP154-21 info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 5.6 2023 CHEMISTRY, ANALYTICAL 12 / 106 = 0.113 2023 Q1 T1 0.956 2023 Analytical Chemistry 2023 Q1 Spectroscopy 2023 Q1 Biochemistry 2023 Q2 12.3 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Cepriá, Gemma (orcid)0000-0003-3727-3275 Camacho-Aguayo, Javier Universidad de Zaragoza (orcid)0000-0003-1654-3803 Martín, Santiago Universidad de Zaragoza (orcid)0000-0001-9193-3874 González Orive, Alejandro de Marcos, Susana Universidad de Zaragoza (orcid)0000-0002-7902-6005 Galbán, Javier Universidad de Zaragoza (orcid)0000-0002-8973-5104 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2012 755 Universidad de Zaragoza Dpto. Química Física Área Química Física 266 (2023), 125119 [6 pp.] Talanta Talanta 0039-9140 3315537 http://zaguan.unizar.es/record/132133/files/texto_completo.pdf Versión publicada 2350023 http://zaguan.unizar.es/record/132133/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:132133 articulos driver 2024-11-22-12:02:18 ARTICLE